Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Biotin-SP (long spacer) IgG Fraction Monoclonal Mouse Anti-Goat IgG, Light Chain Specific

(min X Hrs, Hu, Ms, Rb, Rat Ig)

Target: Goat
Host: Mouse
Antibody Format: IgG Fraction Monoclonal
Specificity: IgG, Light Chain Specific
Minimal Cross Reactivity: Horse, Human, Mouse, Rabbit, Rat Immunoglobulins
Conjugate: Biotin-SP (long spacer)
Product Category: Anti‑Light Chain-Specific Antibodies for Western Blotting after IP
Clonality: Monoclonal

BSA and dry milk may contain IgG which will be recognised by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titre.

Code: 205-062-176

0.5 ml

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Technical Info
Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on goat IgG and with those common to other goat immunoglobulins. The antibody does not react with the heavy chain of goat IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with horse, human, mouse, rabbit and rat immunoglobulins, but it may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solid

Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Biotin-SP (long spacer)

Biotin-SP is our trade name for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.