Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on sheep IgG and with those common to other sheep immunoglobulins. The antibody does not react with the heavy chain of sheep IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, horse, human, mouse, rabbit and rat immunoglobulins, but it may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8° C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol ( ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original.
Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Buffer: 0.01M Tris-HCl, 0.25M NaCl, pH 8.0
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide
Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.
This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.