"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Use the table below to find the secondary antibody you want to pair with our Rabbit Anti-His Tag antibody and include promotion code TAG50 to qualify.
Indirect detection with Jackson ImmunoResearch’s Anti-His Tag antibody in combination with Anti-Rabbit conjugates enhances sensitivity
Signal enhancement can be achieved by indirect detection of the His Tag using an Anti-His Tag antibody followed by a conjugated Anti-Rabbit antibody such as an HRP Goat Anti-Rabbit secondary antibody. Figure 4 shows that sensitivity can be improved by using the indirect two-step method (B) in comparison to direct detection (A).
PolyHistidine Tags (His Tags) are a popular addition to recombinant proteins to facilitate protein purification and detection. His Tags are commonly used in preference to other tags due to the availability of inexpensive purification resins such as NiNTA. Anti-His Tag antibodies enable the detection of the tagged proteins allowing screening of expression.