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"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."Janet Duerr, Ohio University
Congratulations to the winner of our short survey draw at FENS 2018. Associate Professor Dineke Verbeek was the lucky winner of won one of JIR's bespoke leather poster cases. Find out a little about her research below.
Department of Genetics, University of Groningen, The Netherlands
“We aim to better understand the underlying etiology of movement disorders by discovering the genes and biological mechanisms that regulate neurodevelopment and neuronal survival in the brain. We are also exploring how the mutant genes disrupt these mechanisms and lead to disease. Likewise, we aim to identify the shared molecular mechanisms leading to different brain disorders.”
You can find Dineke’s full bio athttps://www.rug.nl/research/genetics/staff/dineke-verbeek
Congratulations to the winner of our short survey draw at ECI 2018. Carina won one of JIR's bespoke leather poster cases. Find out a little about her research.
Carina Miggitsch is a PhD student working in the immunology group of Beatrix Grubeck-Loebenstein in Innsbruck. She is focusing on the characterization of human bone marrow adipocytes and investigate their positive or negative effect on immune cells, especially effector/memory T cells. She was nominated for the 2018 ECI poster award candidate prize, but unfortunately did not win the poster prize on this occasion. Carina told JIR “That's why I was even more excited when I read that I won this nice vintage poster case. Thank you so much!”
Congratulations to the winner of our short survey draw at
University of Duisburg-Essen and University Clinic Essen, Germany
Sophie started her PhD in December 2016 at the Institute of Experimental Immunology and Imaging at the University of Duisburg-Essen and University Clinic Essen, having also completed her bachelor's and master's degree in biology at the University.
"My main topic of research is immunology. I look at the response of different immune cells to lung infections in mice, most of the time with an intravital imaging method but also on stained cryo-sections.
Congratulations to the winner of our short survey draw at the FENS Regional meeting in Pécs, Hungary 2017 - “what do you do with your secondary antibodies?”. Find out a little about her work using Jackson ImmunoResearch Secondary Antibodies.
University of Pécs, Hungary
Dr Kovács-Valasek's Lab is currently working on mammalian neurogenesis.
"Our lab are interested in elucidating the effect of microRNA in the process of mammalian neurogenesis especially during the first three postnatal period. Therefore, we are using a lot of primary and secondary antibodies for in situ microRNA hybridisation combined with immunohistochemical-labeling, and for Western blot detection."
Congratulations to the winner of our short survey draw at NeuroFrance 2017 - “what do you do with your secondary antibodies?”. Find out a little about her work using Jackson ImmunoResearch Secondary Antibodies.
University Pierre et Marie Curie, Paris
"One of the topics in the lab is to understand the role of the glutamate receptors of the delta family in the brain. I am currently using co-immunoprecipitation and western blots to investigate the GluD1 interactome and signaling. I also use immunolabelling of brain slices and cell culture to examine the distribution of GluD1 and its partners. I am very happy to use the fluorescent JIR antibodies suitable for the LiCor Odyssey detection system and directed against the IgG light chains for my co-IP experiments. Of course, many other JIR antibodies are present in our antibody toolbox."
Congratulations to the winner of our short survey draw at the German Neuroscience meeting in Göttingen 2017 - “what do you do with your secondary antibodies?”. Find out a little about her work using Jackson ImmunoResearch Secondary Antibodies.
Charité University of Medicine in Berlin
Ira studied cell biology in Osnabrück (Germany), specialising in neurobiology and developmental biology. Find out more about her work and how she uses Jackson ImmunoResearh secondary antibodies.
"Since 2014 I'm doing my PhD with help of the Charité Berlin in the otolaryngology at the Unfallkrankenhaus (accidental hospital) Berlin. There I investigate a near-infrared laser pre-treatment as a protective mechanism to prevent sensorineural hearing damage due to traumatic interventions of the cochlea, since it is known that after noise traumas not only the peripheral but especially the basal central structures and thus the physiology of auditory perception are significantly changed. It is important for me to get results from immunohistological stainings with help of your antibodies like AlexaFluor for counting hair cells in the cochlea after pre-treatment and noise exposure."
Congratulations to the winner of our short survey draw at SFI 2016 - “what do you do with your secondary antibodies?”. Find out a little about his work using Jackson ImmunoResearch Secondary Antibodies.
3rd year PhD student
"Our team is focused on Inflammatory Bowel diseases (IBD). Using different approaches, we aim to clearly identify the sources and functions of a specific molecule expressed in rat intestine that my represent a potential for therapeutic.During my PhD, I perform Flow cytometry and immunofluorescence staining using a lot of Jackson ImmunoResearch secondary antibodies that represent a standard of specificity in our laboratory."
Laboratory of Growth Control and Cancer Research
Department of Oncology
“In our lab we are focusing on the Hippo signaling pathway and its role in animal organ growth control and oncogenesis. In my PhD project I am working on identifying and characterizing new components of the Hippo pathway using Drosophila as my animal model. Acquiring nice confocal images of my samples is my favourite part of the job. My research largely depends on good antibody stainings. A significant part of the secondary antibodies we use in our lab come from Jackson ImmunoResearch. I use these reagents on weekly basis and I can hardly imagine my research without them.”
Figure: Control RNAi (Top) and warts (wts) RNAi (bottom) co-expressed with GFP in the Drosophila wing imaginal disc. Expanded (ex) lacZ is the activity readout for the Hippo pathway effector Yorkie. Depletion of the Warts protein in the GFP positive region results in tissue overgrowth and induction of the Yorkie activity reporter ex-lacZ. Cy3 anti-mouse from Jackson ImmunoResearch was used to stain for beta-galactosidase to visualise ex-lacZ.
The winner of our short survey draw at DGFI 2016 - “what do you do with your secondary antibodies?” was lucky enough to win an iPad mini.
Institute for Virology
University Hospital Essen
The winner of our short survey draw at EMBO 2016 - “what do you do with your secondary antibodies?” was lucky enough to win an iPad mini.
Developmental and Cell Biology Department,
Faculty of Science,
Prague, Czech Republic
"My research is currently focusing into Epithelial- mesenchymal transition (EMT) and differentiation potential of EMT- derived cells from testis. Therefore, we are using a lot of primary antibodies, and secondary antibodies as well. Secondary antibodies from Jackson ImmunoResearch are always my first choice because of their specificity, and especially because of the useful technical support from them."
The winner of our short survey draw at ICCB 2016 - “what do you do with your secondary antibodies?” was lucky enough to win an iPad mini. Find out a little about his work below.
“My research tries to understand how elaborate and highly accurate microtubule rearrangements are driven in plant cells by developmentally or conditionally important cell signaling. Currently I am focused on mitogen activated protein kinases and their functional and physical interactions with effectors of microtubule dynamics and organization using the model plant Arabidopsis thaliana. I employ biochemistry, molecular biology and genetics and I combine them with different microscopic imaging strategies.”
During FENS 2016 we ran our short survey “what do you do with your secondary antibodies?” The winner was lucky enough to win an iPad mini.
Here’s a little about the winner and his work.
"Our group is primarily focused on Huntington's Disease (HD). Having a multidisciplinary approach, we cover a broad range of interest in HD research; ranging from behavioural deficits in HD; basic physiopathology at biochemical, and bio-molecular levels; new biomarkers for disease onset follow-up, new pathogenic mechanisms; and of course, potential therapeutic routes and approaches including improving neurotrophic support, stem cell and gene therapy, etc. My PhD project is focused on using drugs with potential to improve neurotrophic support in the striatum which is the region earliest and most severely affected in HD. We have described possible uses of known drugs as HD therapies, and to evaluate that we focus on the effect in neuronal branching of compounds compared to known neurotrophin BDNF, to perform these studies we perform immunofluorescence staining which includes exclusively Jackson ImmunoResearch secondary antibodies (Cy3, AlexaFluor 488 etc.) which are the standard in our laboratory."
During 2015 we ran a wordsearch puzzle competition at scientific exhibitions and meetings, we entered all correct entries received into an iPad prize draw.
"In my group we are specially interested in elucidating the effects of TGF-beta in liver physiology and pathology. In particular, my project is focused on the effects that the protein caveolin-1 exerts on the cross-talk between TGF-beta and EGFR signaling pathways in hepatocytes and hepatocellular carcinoma cells."
To thank those who participated in our customer service survey in Spring 2015, we entered all responses received into an iPad prize draw.
"Our lab interest revolves around the structure of chromatin in 3d nuclear space and time. In order to unravel the association of transcription and chromatin structure over time, our lab has adopted as a model the cytokine transcriptional regulation. Before we engage in various next generation techniques, such as ChiP-seq or Hi-C we verify system activation. One way is to study key transcription factors shuttling upon cytokine activation. FITC- or Rhodamine-TRITC-conjugated secondary antibodies from Jackson ImmunoResearch were used to detect on immunofluorescence experiments the translocation to the nucleus of key transcription factors, like those of the SMAD superfamily."
As part of our celebrations for the 10th anniversary JIR-E, the European subsidiary of Jackson ImmunoResearch Laboratories, inc. we awarded sponsorships of €500 to researchers who published during 2014 using our secondary antibodies.
"Our lab does electrophysiology in the turtle spinal cord. To visualize and characterize the neurons that are measured we pump biotin into the measured cell and stain slices of the spinal cord with Streptavidin-Cy3 (016-160-084) and identify the motor neuron by staining for choline acetyltransferase (ChAT) and using anti-goat secondary antibodies conjugated to Alexa Fluor 488® (705-545-147).
Our work is featured on the front cover of the Journal of Neuroscience, February 19th 2014, where ChAT positive motor neurons are green, the measured cell red and blue for DAPI. The secondary antibody allows us to not only to stain the cell body, but also to trace the fine filaments of the motor neurons. Shown here is one of my favorite images of the ventral horn with the motor neuron population.
The low cross reactivity and the broad selection of targets and conjugates gives a great flexibility when doing multiple labeling, especially in an odd species like the turtle."
"We recently published our work in the Journal of Cell Biology with the title "Chk2 prevents mitotic exit when the majority of kinetochores are unattached" with Pubmed ID 24798733. We used FITC- or Rhodamine-TRITC-conjugated secondary antibodies from Jackson ImmunoResearch in Immunofluorescence:"
|515-025-072||TRITC Sheep Anti-Mouse IgG, F(ab')₂ Fragment Specific|
|111-025-046||TRITC Goat Anti-Rabbit IgG, Fc Fragment Specific|
|115-096-072||FITC F(ab')₂ Fragment Goat Anti-Mouse IgG, F(ab')₂ Fragment Specific|
|713-095-147||FITC Donkey Anti-Sheep IgG (H+L)|
|111-096-047||FITC F(ab')₂ Fragment Goat Anti-Rabbit IgG, F(ab')₂ Fragment Specific|
"We only use secondary antibodies from JIR because they are very specific and have low background and cross-reactivity."
Well done to everyone who correctly identified "Normal Serum" as the correct answer. The winning entry was from Wojciech J. Szlachcic from the Institute of Bioorganic Chemistry, Polish Academy of Sciences, who received an iPad:
"In my research I work with induced pluripotent stem cells for modelling and cell therapy treatment of Huntington's disease. I'm also involved in a project aimed at discovery of pathogenesis mechanisms in MJD/SCA3 using Ki91 knock-in mouse model of the disease. I always use JIR's normal serums and secondary antibodies for immunofluorescence and western blot assays."