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The epidermis was separated from dermis by creating a suction blister. The flattened blister roof was then processed without sectioning and stained for localization of nerve endings (Cy3, recolored green) and Langerhans cells (Cy2, recolored red) using Cy2- and Cy3- conjugated secondary antibodies from Jackson ImmunoResearch Laboratories, Inc. to provide a "bird's eye view" of the distribution of nerve endings and dendritic cells within the epidermis. Some basal cells are weakly positive for the nerve marker.
Image contributed by Dr. William R. Kennedy and Dr. Gwen Wendelschafer-Crabb, Department of Neurology, University of Minnesota.