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FISH signal from transgenic tobacco (Nicotiana tabacum) containing four copies of the pine drought-inducible lp5 gene. Using karyotypic features to help identify chromosome homologues, one locus (1) is homozygous, and two are heterozygous (2 and 3). The nontransgenic homologue of 2 is karyotypically identifiable [(2)], whereas that of 3 is not unequivocally so. The results illustrate feasibility of routine detection of alien transgenes inserted into eukaryotic genomes.
Signal was detected with sequential applications of mouse anti-biotin and Cy3-conjugated goat anti-mouse antibodies. Chromosomes were counterstained with DAPI.
The photograph was taken on an AX-70 Olympus (ASA 400 film). From collaborative efforts of Dr. M. Nurul Islam-Faridi, Dr. David Stelly, Dr. H. James Price, Dr. Jean Gould, Dr. Ronald Newton, Texas A&M University, College Station, TX, USA. Image is for viewing only; do not reproduce. Photo submitted by Dr. David Stelly, Texas A&M University.