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Antibodies for Serological Testing

Anti-Human Antibodies

Anti-Human IgG
Anti-Human IgM
Anti-Human IgA

Available in single and combined specificities, unconjugated and conjugated formats, including HRP and Biotin.

Manufactured under ISO 9001:2015 certification.

Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

Rating: 5.0

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Suggested Dilution Factors

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Suggested dilution factors

The dilution factors suggested in the following table are presented as ranges because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The optimal working dilution should be determined empirically for each application.

		Application
Product	Conjugate	ELISA	Western Blots	Histo-/Cyto chemistry	Flow Cytometry
Whole IgG and F(ab')₂ secondary antibodies	Unconjugated	10-20 µg/ml
Fab secondary antibodies	Unconjugated	20-40 µg/ml
Whole IgG, F(ab')₂ and Fab fragment secondary antibodies	Alexa Fluor® 488, 594, 647, DyLight™ 405, and Cy™3	1:100-1:800
Whole IgG secondary antibodies	Alexa Fluor® 680 and 790		1:50,000-1:200,000
Whole IgG, F(ab')₂ and Fab fragment secondary antibodies	AMCA, BV421™, BV480™, Cy2, FITC, TRITC, RRX, and Texas Red	1:50-1:200
Whole IgG secondary antibodies	Cy™5	1:100-1:400
Whole IgG, F(ab')₂ secondary antibodies and Streptavidin	R-PE and APC				1:50-1:200
Whole IgG, F(ab')₂ secondary antibodies and Streptavidin	PerCP				1:25-1:100
Whole IgG and F(ab')₂ secondary antibodies	Horseradish Peroxidase	1:5,000-1:100,000	1:5,000-1:100,000 (non-ECL) 1:10,000-1:200,000 (ECL)	1:500- 1:5,000
Whole IgG and F(ab')₂ secondary antibodies	Alkaline Phosphatase	1:5,000-1:50,000	1:5,000-1:50,000
Whole IgG, F(ab')₂ and Fab secondary antibodies	Biotin-SP (using Fluorophore-Conjugated Streptavidin)			1:200-1:1,000	1:200-1:1,000
Whole IgG and F(ab')₂ secondary antibodies	Biotin-SP (using enzyme-Conjugated Streptavidin)	1:20,000-1:400,000	1:20,000-1:400,000	1:500-1:5,000
Streptavidin	Horseradish Peroxidase	1-2 µg/ml	0.01-0.1 µg/ml	1-2 µg/ml
Streptavidin	Alkaline Phosphatase	1-2 µg/ml	0.1-1 µg/ml	1-2 µg/ml
Streptavidin	Alexa Fluors®, DyLight 405, and Cy3		0.5-2 µg/ml	0.5-2 µg/ml
Streptavidin	Cy5		0.5-2 µg/ml	0.5-2 µg/ml
Streptavidin	All Other Fluorophores		2-5 µg/ml	2-5 µg/ml
4 nm Gold-Antibody Complexes				1:20-1:200
6, 12 nm Gold-Antibody Complexes				1:20-1:40
18 nm Gold-Antibody Complexes				1:10-1:20
Peroxidase-Anti-Peroxidase (PAP)				25-50 µg/ml
FabuLight™ Anti-IgM		To complex with primary antibody in solution, use 1:1 weight ratio of Fab: primary antibody (15:1 molar ratio).
FabuLight Anti-IgG		To complex with primary antibody in solution, use 1:1 weight ratio of Fab: primary antibody (3:1 molar ratio).
Normal Serum		5% (v/v) for blocking
ChromPure™		10 µg/ml