Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

Find By Code Number

Product Filter

Spotlight on...

Fluorescent Dyes

Brilliant Violet™
421 & 480

  • Combine with AF488, RR-X, & AF647 for 5-color imaging
  • Compatible with commonly used filter sets
  • Switch nuclear stain from DAPI to DRAQ5™ for additional labeling options

Learn More

Whole IgG Affinity-Purified Secondary Antibodies

"Without question, I can always turn to Jackson ImmunoResearch secondary antibodies for ECL detection of any primary antibody! They produce great signal at low dilutions."

W Thompson, OSHU

Leave a Review

FabuLight - Fab Labeling Example Protocol

FabuLight™ Example Protocol - Fab Labeling Primary Antibodies

1. Include a blocking step if the tissue displays immunoglobulins that could be recognized by the FabuLight, for instance mouse tissue that is to be labeled with a mouse specific FabuLight. Otherwise start with step 2.
Incubate with unconjugated Fab anti-Fc antibody, in this example Fab Goat Anti-Mouse IgG1, Fcγ fragment specific. Fab Goat Anti-Mouse IgG (H+L) could also be used for this step. Wash.

2. Create Fab-primary antibody complexes. In this example Mouse Anti-Antigen X is complexed with Alexa Fluor® 488-conjugated Fab Goat Anti-Mouse IgG1, Fcγ fragment specific (Creating Alexa Fluor® 488-labeled Anti-X Complex); and Mouse Anti-Antigen Y is complexed with Rhodamine Red-X-conjugated Fab Goat Anti-Mouse IgG1, Fcγ fragment specific (Creating Rhodamine Red-X-labeled Anti-Y Complex).

3. Incubate the sample with Alexa Fluor® 488-labeled Anti-X Complex. (X being the less abundant target) Wash.

4. Incubate the sample with Rhodamine Red-X-labeled Anti-Y Complex. (Y being the more abundant target) Wash.

Follow the steps above for an example protocol using Fab-labeled primaries for labeling two antigens on tissue.

Browse FabuLight - Affinity-Purified Fab Anti-Fc Fragment Specific Antibodies

Download White Paper - FabuLight Fab labeling primary antibodies with fluorophore-conjugated Fab anti-Fc