Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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FabuLight - Fab Labeling Example Protocol

FabuLight™ Example Protocol - Fab Labeling Primary Antibodies

1. Include a blocking step if the tissue displays immunoglobulins that could be recognized by the FabuLight, for instance mouse tissue that is to be labeled with a mouse specific FabuLight. Otherwise start with step 2.
Incubate with unconjugated Fab anti-Fc antibody, in this example Fab Goat Anti-Mouse IgG1, Fcγ fragment specific. Fab Goat Anti-Mouse IgG (H+L) could also be used for this step. Wash.

2. Create Fab-primary antibody complexes. In this example Mouse Anti-Antigen X is complexed with Alexa Fluor® 488-conjugated Fab Goat Anti-Mouse IgG1, Fcγ fragment specific (Creating Alexa Fluor® 488-labeled Anti-X Complex); and Mouse Anti-Antigen Y is complexed with Rhodamine Red-X-conjugated Fab Goat Anti-Mouse IgG1, Fcγ fragment specific (Creating Rhodamine Red-X-labeled Anti-Y Complex).

3. Incubate the sample with Alexa Fluor® 488-labeled Anti-X Complex. (X being the less abundant target) Wash.

4. Incubate the sample with Rhodamine Red-X-labeled Anti-Y Complex. (Y being the more abundant target) Wash.

Follow the steps above for an example protocol using Fab-labeled primaries for labeling two antigens on tissue.

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Download White Paper - FabuLight Fab labeling primary antibodies with fluorophore-conjugated Fab anti-Fc