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"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio University
IgG fraction Monoclonal Mouse Anti-Biotin, Anti-Fluorescein, and Anti-Digoxin may be used either as direct conjugates, or for more sensitivity, they can be used unconjugated followed by a conjugated anti-mouse IgG (H+L) for signal enhancement (see examples below using Mouse Anti-Biotin).
Our monoclonal Mouse Anti-Biotin antibody provides an alternative to streptavidin for amplification procedures. It can be used for in situ hybridization, immunohistochemistry, and flow cytometry. Two examples of such amplification are shown below.
These images are for viewing only, please do not reproduce. FISH of 75 kb low-copy genomic BAC (bacterial artificial chromosome) clone of cotton (Gossypium hirsutum) to root-tip preparations from 26-chromosome relative, G. arboreum.
Signal was detected with sequential applications of mouse anti-biotin and Cy3-conjugated goat anti-mouse antibodies. Chromosomes were counterstained with DAPI. Photomicrographs were taken on Fujichrome 400, using an Olympus AX-70.
BAC FISH sites are visible in this interphase nucleus. Examples of applications include checking for chimerism among BAC libraries, and assembly of integration maps (physical and genetic).
Two pairs of signals, one per chromatid, are visible in this meristem mitotic, metaphase chromosome spread.
Images are from collaborative efforts of Dr. Rob Hanson, Dr. M. Nurul Islam-Faridi, Dr. David Stelly, and the BAC Center, Texas A&M University. Photos submitted by Dr. Stelly, Texas A&M University. These images are for viewing only; please do not reproduce.
Affinity-purified anti horseradish peroxidase (HRP) may be used to detect HRP or to enhance signal by binding to HRP-conjugated molecules (such as HRP-conjugated antibodies or HRP-conjugated streptavidin) or HRP-containing complexes (such as PAP or HRP-ABC). Anti-HRP also may be used to convert an HRP conjugate into a different signal. For example, our anti-HRP complexed with colloidal gold particles has been used with silver enhancement in place of diaminobenzidine (DAB) to create less diffused images following labeling of tissue sections with HRP reagents (Gee et al., J. Histochem. Cytochem. 1991. 39, 863; Roth et al., Methods in Lab. Invest. 1992. 67, 263; Roth et al., Histochemistry. 1992. 98, 229). A further advantage of the technique was a reduction in background staining from endogenous peroxidase-like enzymes in animal tissue. The background, (typically detected with DAB), was not detected with anti-HRP, since HRP is a plant enzyme.