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"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."Janet Duerr, Ohio University
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Monoclonal Mouse Anti-Biotin, Anti-Fluorescein and Anti-Digoxin are available in a wide range of conjugate options for direct detection of their target molecules, which are commonly used as tags on proteins and nucleic acids. If signal amplification is desired, these antibodies can be used unconjugated, followed by conjugated anti-mouse IgG (H+L) (see examples below using Mouse Anti-Biotin).
Affinity-purified anti-horseradish peroxidase (HRP) may be used to detect HRP or to enhance signal by binding to HRP-conjugated molecules. Anti-HRP also may be used to convert an HRP conjugate into a different signal as illustrated in the example below using ImmunoGold-complexed anti-HRP. Goat Anti-HRP is also available conjugated to alkaline phosphatase and fluorescent probes, providing additional flexibility to the detection method.