Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Fluorescent Dyes

Brilliant Violet™
421 & 480

  • Combine with AF488, RR-X, & AF647 for 5-color imaging
  • Compatible with commonly used filter sets
  • Switch nuclear stain from DAPI to DRAQ5™ for additional labeling options

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Whole IgG Affinity-Purified Secondary Antibodies

"Without question, I can always turn to Jackson ImmunoResearch secondary antibodies for ECL detection of any primary antibody! They produce great signal at low dilutions."

W Thompson, OSHU

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Mouse Subclass Specific Secondary Antibodies

Anti-Mouse IgG Subclass Specific Secondary Antibodies

Mouse IgG subclasses

Mice express 4 of the 5 available IgG subclasses making up their IgG isotype. They typically encode for IgG1, IgG2b and IgG3, and depending on their strain will also express either IgG2a or IgG2c (Collins 2016). IgG2a and IgG2c subclasses are seen as functionally comparable, being the most active of the subclasses to bind complement (Collins 2016).

Inbred mouse strains, such as C57Bl/6, C57Bl/10, SJL and NOD mice, possess an IgH-1b allele which results in expression of IgG2c instead of the IgG2a subclass expressed by BALB/c and Swiss Webster mice. The IgH-1a haplotype of these mice strains include the IGHG2C gene alongside the other isotypes, but not the IGHG2A present on the IgH-1b haplotype of the BALB/c mice (Martin et al 1998).

A number of monoclonal antibodies originate from these inbred stains, and some IgG2c clones have been incorrectly isotyped as IgG2a by reagents that cannot distinguish between these two subclasses. Subclass specific antibodies from Jackson ImmunoResearch provide exquisite discrimination among the subclasses.

Distinguish between different mouse IgG subclasses

Jackson ImmunoResearch anti-mouse IgG, subclass specific antibodies offer specificity to the 5 individual mouse IgG subclasses. These highly specific antibodies are designed to distinguish between two or more different subclass of mouse IgG in multiple labeling experiments, or for mouse IgG subclass determination.

They have been adsorbed against human, bovine and rabbit serum proteins to minimize interference from cross-reactivity with tissue immunoglobulins, adherent bovine IgG on cultured cells and rabbit primary antibodies.

Anti-Mouse IgG, subclass specific antibodies are available conjugated to Alexa Fluor® and Cyanine™ Fluorescent dyes, Biotin-SP™, and horseradish peroxidase and alkaline phosphatase reporter molecules.


The example below illustrates the performance of the Goat Anti-Mouse subclass specific antibodies in a dot blot experiment. In each case, the subclass specific antibody only recognizes its specific target in the presence of additionally blotted subclasses.




Figure 2: Dot blot showing the specificity of goat anti-mouse IgG, Fcγ subclass specific antibodies. Separate nitrocellulose strips (rows) received 100 ng “dots” of mouse IgG and each subclass, and then were blocked with 5% (w/v) BSA in PBST. After probing with Peroxidase-conjugated goat anti-mouse subclass specific antibodies, the strips were developed with TMBM substrate from Moss, Inc. The grid of positive signals shows the specificity of each subclass directed antibody. Some subclasses are poorly represented in a total IgG pool (IgGtotal, α-IgGs 2c and 3) and thus give weak signal. Peroxidase conjugates used for probing were 115-035-205 (anti-mouse IgG1), 115-035-206 (anti-mouse IgG2a), 115-035-207 (anti-mouse IgG2b), 115-035-208 (anti-mouse IgG2c), and 115-035-209 (anti-mouse IgG3).


*Subclass specific antibodies are not necessary for general detection of mouse monoclonal antibodies in single-labeling experiments or in multiple-labeling experiments involving one mouse monoclonal and primary antibodies from other species.


Browse secondary antibodies by subclass

IgG1 IgG2a IgG2b IgG2c IgG3


References:

Collins, A. (2016). IgG subclass co-expression brings harmony to the quartet model of murine IgG function. Immunol Cell Biology, 94, 10, 949- 954. http://dx.doi.org/10.1038/icb.2016.65

Martin, R., Brady, J., Lew, A. (1998) The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice, Journal of Immunological Methods, Volume 212, Issue 2, 1998, Pages 187-192, ISSN 0022-1759.  http://dx.doi.org/10.1016/S0022-1759(98)00015-5

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AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific

Antibody Description Product Code
AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-005-205
Peroxidase-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-035-205
Alkaline Phosphatase-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-055-205
Biotin-SP-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-065-205
DyLight 405-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-475-205
Brilliant Violet™ 421-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-675-205
AMCA-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-155-205
Brilliant Violet™ 480-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-685-205
Cy2-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-225-205
Alexa Fluor® 488-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-545-205
FITC-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-095-205
Cy3-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-165-205
R-Phycoerythrin-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-115-205
Rhodamine Red-X-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-295-205
Alexa Fluor® 594-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-585-205
Allophycocyanin-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-135-205
Cy5-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-175-205
PerCP-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-125-205
Alexa Fluor® 680-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-625-205
Alexa Fluor® 790-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-655-205


AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific

Antibody Description Product Code
AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-005-206
Peroxidase-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-035-206
Alkaline Phosphatase-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-055-206
Biotin-SP-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-065-206
DyLight 405-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-475-206
Brilliant Violet™ 421-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-675-206
AMCA-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-155-206
Brilliant Violet™ 480-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-685-206
Cy2-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-225-206
Alexa Fluor® 488-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-545-206
FITC-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-095-206
Cy3-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-165-206
R-Phycoerythrin-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-115-206
Rhodamine Red-X-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-295-206
Alexa Fluor® 594-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-585-206
Allophycocyanin-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-135-206
Alexa Fluor® 647-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-605-206
Cy5-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-175-206
PerCP-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-125-206
Alexa Fluor® 680-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-625-206
Alexa Fluor® 790-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-655-206


AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific

Antibody Description Product Code
AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-005-207
Peroxidase-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-035-207
Alkaline Phosphatase-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-055-207
Biotin-SP-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-065-207
DyLight 405-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-475-207
Brilliant Violet™ 421-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-675-207
AMCA-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-155-207
Brilliant Violet™ 480-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-685-207
Cy2-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-225-207
Alexa Fluor® 488-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-545-207
FITC-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-095-207
Cy3-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-165-207
R-Phycoerythrin-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-115-207
Rhodamine Red-X-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-295-207
Alexa Fluor® 594-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-585-207
Allophycocyanin-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-135-207
Alexa Fluor® 647-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-605-207
Cy5-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-175-207
PerCP-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-125-207
Alexa Fluor® 680-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-625-207
Alexa Fluor® 790-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-655-207


AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific

Antibody Description Product Code
AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-005-208
Peroxidase-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-035-208
Alkaline Phosphatase-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-055-208
Biotin-SP-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-065-208
DyLight 405-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-475-208
AMCA-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-155-208
Alexa Fluor® 488-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-545-208
FITC-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-095-208
Cy3-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-165-208
R-Phycoerythrin-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-115-208
Rhodamine Red-X-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-295-208
Alexa Fluor® 594-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-585-208
Allophycocyanin-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-135-208
Alexa Fluor® 647-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-605-208
PerCP-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-125-208


AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific

Antibody Description Product Code
AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-005-209
Peroxidase-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-035-209
Alkaline Phosphatase-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-055-209
Biotin-SP-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-065-209
DyLight 405-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-475-209
Brilliant Violet™ 421-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-675-209
AMCA-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-155-209
Brilliant Violet™ 480-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-685-209
Cy2-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-225-209
Alexa Fluor® 488-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-545-209
FITC-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-095-209
Cy3-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-165-209
R-Phycoerythrin-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-115-209
Rhodamine Red-X-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-295-209
Alexa Fluor® 594-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-585-209
Allophycocyanin-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-135-209
Alexa Fluor® 647-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-605-209
Cy5-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-175-209
PerCP-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-125-209
Alexa Fluor® 680-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass c Specific
(min X Hu,Bov,Rb Sr Prot)
115-625-209
Alexa Fluor® 790-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-655-209