Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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DyLight™ 549 AffiniPure Fab Fragment Goat Anti-Human IgM, Fc fragment specific


Target: Human
Host: Goat
Antibody Format: Fab Fragment
Specificity: IgM, Fc fragment specific
Conjugate: DyLight™ 549
Product Category: Fab Fragment Antibody Conjugates With Limited Stock
Clonality: Polyclonal
RRID: AB_2337818

Discontinued - Limited Stock Available

Code: 109-507-043

Unit:
1.0 mg
Price:
$281.00



Technical Info

Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.

Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of the human IgM heavy chain but not with human IgG, IgA, or the light chains of human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with IgM from other species.
Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

1:100 - 1:800 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

DyLight™ 549

Amax: 555 Emax: 568nm

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

DyLight™ is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

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