Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Lateral Flow Tests

Lateral Flow: Key of Elements.
Lateral Flow: Key of Elements.
Lateral Flow: step one.

Step 1. Lateral flow test strips commonly consist of a sample pad, a conjugate pad that has been impregnated with conjugated antibodies, for example, gold conjugated Goat Anti-Human Fcγ specific antibodies and two “lines” which have been applied to nitrocellulose with antigen (T), and control antibodies, for example, Bovine Anti-Goat (C).

Lateral Flow: step two.

Step 2. The sample is introduced to the sample pad via a hole in the test strip cassette.

Lateral Flow: step three.

Step 3. Capillary action draws the sample into the conjugate pad and rehydrates the impregnated antibody conjugates. Any antibodies in the sample to which the conjugated antibody is specific will form complexes.

Lateral Flow: step four.

Step 4. The sample and complexed antibodies migrate onto the antigen-coated nitrocellulose (T) test “line” where they interact.

Lateral Flow: step five.

Step 5. If the antibodies in the sample are specific to the coated antigen the antibody complexes will be deposited and develop color over the line giving a visual indication of a “positive” test. If there are no antibodies specific to the antigen in the test sample, no colored line will develop. Un-complexed gold conjugated Goat Anti-Human antibodies continue to migrate through the test strip to the control line (C).

Lateral Flow: step six.

Step 6. The free conjugated Goat Anti-Human antibodies can be captured by the coated Bovine Anti-Goat IgG and develop a colored line to indicate the test has been performed correctly. No control line would indicate an invalid test.

Lateral Flow

Rapid assays like lateral flow tests provide quick yes or no results in an easy to use format. They allow for point of care analysis as an alternative to more time-consuming immunoassays that need to be performed in laboratories. Rapid tests are used to qualitatively detect patient-generated antibodies to enable clinical diagnosis and indicate treatment.

How do they work?

Lateral flow or rapid tests use the principle of immunochromatography - whereby the components of the test flow laterally through a matrix by capillary action allowing the antigens to combine with antibodies to facilitate the detection and visualization of those complexes.

Lateral Flow: step one. Lateral Flow: step two. Lateral Flow: step three. Lateral Flow: step four. Lateral Flow: step five. Lateral Flow: step six.

Step 1. Lateral flow test strips commonly consist of a sample pad, a conjugate pad that has been impregnated with conjugated antibodies, for example, gold conjugated Goat Anti-Human Fcγ specific antibodies and two “lines” which have been applied to nitrocellulose with antigen (T), and control antibodies, for example, Bovine Anti-Goat (C).

Step 2. The sample is introduced to the sample pad via a hole in the test strip cassette.

Step 3. Capillary action draws the sample into the conjugate pad and rehydrates the impregnated antibody conjugates. Any antibodies in the sample to which the conjugated antibody is specific will form complexes.

Step 4. The sample and complexed antibodies migrate onto the antigen-coated nitrocellulose (T) test “line” where they interact.

Step 5. If the antibodies in the sample are specific to the coated antigen the antibody complexes will be deposited and develop color over the line giving a visual indication of a “positive” test. If there are no antibodies specific to the antigen in the test sample, no colored line will develop. Un-complexed gold conjugated Goat Anti-Human antibodies continue to migrate through the test strip to the control line (C).

Step 6. The free conjugated Goat Anti-Human antibodies can be captured by the coated Bovine Anti-Goat IgG and develop a colored line to indicate the test has been performed correctly. No control line would indicate an invalid test.

Lateral Flow: Key of Elements.
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