"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio UniversityRating: 5.0
Allophycocyanin (APC) is an approximately 105 kDa phycobiliprotein containing the chromophore phycocyanobilin, isolated from the blue-green alga Spirulina. APC is chemically cross-linked to stabilize the constituent subunits making up the molecule. After phycobiliproteins are conjugated to secondary antibodies, there is little fluorescence quenching, which results in conjugates of high specific fluorescence compared with conventional fluorophore-antibody conjugates. APC can be excited by light over a wide range of the visible spectrum, is highly water soluble, has a relatively low isoelectric point, and lacks potentially sticky carbohydrates. APC conjugates are suitable for cell surface labeling techniques such as flow cytometry.
APC is available conjugated to:
| Excitation Peak (nm) | Emission Peak (nm) | |
|---|---|---|
| Allophycocyanin APC | many, 650 | 660 |
| Control Reagents | Product Code |
|---|---|
| Allophycocyanin (APC) ChromPure Donkey IgG, F(ab')2 fragment | 017-130-006 |
| Allophycocyanin (APC) ChromPure Goat IgG, whole molecule | 005-130-003 |
| Allophycocyanin (APC) ChromPure Goat IgG, F(ab')2 fragment | 005-130-006 |
| Streptavidin | Product Code |
| Allophycocyanin (APC) Streptavidin | 016-130-084 |
Use the spectra viewer below to compare APC with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.
