Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Multiple Labeling

With BV421™ & BV480™

  • Combine with AF488, RR-X, and AF647 for 5-color imaging
  • Compatible with commonly used filter sets
  • Switch nuclear stain from DAPI to DRAQ5 for additional labeling options

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Allophycocyanin APC

Allophycocyanin APC Technical Information

Allophycocyanin APC is among several kinds of light-harvesting phycobiliproteins found in red, blue-green, and cryptomonad algae. It is isolated from the blue-green alga Spirulina, and is chemically cross-linked for stability. After phycobiliproteins are conjugated to secondary antibodies, there is little fluorescence quenching, which results in conjugates of high specific fluorescence compared with conventional fluorophore-antibody conjugates. APC can be excited by light over a wide range of the visible spectrum, is highly water soluble, has a relatively low isoelectric point, and lacks potentially sticky carbohydrates.

APC Spectra Excitation and emission spectra of APC-conjugated affinity-purified secondary antibodies, streptavidin, and purified proteins. Peak heights have been normalized, spectra were obtained with an M-Series spectrophotometer system from Photon Technology International, Inc. Values are approximate, actual values may vary depending on the spectrofluorometer used in each laboratory.

It should be noted that the relatively high molecular weight of APC may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.

Excitation Peak Emission Peak (nm)
Allophycocyanin APC many, 650 660
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