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Allophycocyanin APC is among several kinds of light-harvesting phycobiliproteins found in red, blue-green, and cryptomonad algae. It is isolated from the blue-green alga Spirulina, and is chemically cross-linked for stability. After phycobiliproteins are conjugated to secondary antibodies, there is little fluorescence quenching, which results in conjugates of high specific fluorescence compared with conventional fluorophore-antibody conjugates. APC can be excited by light over a wide range of the visible spectrum, is highly water soluble, has a relatively low isoelectric point, and lacks potentially sticky carbohydrates.
It should be noted that the relatively high molecular weight of APC may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.
|Excitation Peak||Emission Peak (nm)|
|Allophycocyanin APC||many, 650||660|