Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

4 nm Colloidal Gold AffiniPure™ Goat Anti-Human Serum IgA, α chain specific (LM Grade)

Target: Human
Host: Goat
Antibody Format: Whole IgG
Specificity: Serum IgA, α chain specific
Conjugate: 4 nm Colloidal Gold
Product Category: Anti-Human Antibodies For Diagnostics
Clonality: Polyclonal
RRID: AB_2337743

Suitable for multiple labeling (see our Multiple Labeling guidance)

Code: 109-185-011

1.0 ml

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Technical Info
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of human IgA but not with human IgG or IgM. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with IgA from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use.
Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Borate - Sodium Phosphate, 0.15M NaCl, pH 8.5
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:
Histo-/Cyto-Chemistry:- 1:20-1:200

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

4 nm Colloidal Gold

LM Grade colloidal gold reagents are intended for light microscopy and immunoblotting. Since signal intensity is relatively independent of particle size when silver enhancement is used, we offer 4 nm particles because this size may penetrate tissues better than larger particles.

The 4 nm size may be used for electron microscopy in studies that require smaller particles since they are relatively uniform in size (coefficient of variation less than or equal to 15%), though small aggregates are not removed from this grade. Therefore, our 4 nm particles should only be used for electron microscopy with the
understanding that the presence of small aggregates may give misleading results.

A detailed protocol for silver enhancement is provided with all orders for LM Grade products. All reagents involved in the light-insensitive silver enhancement reaction can be prepared easily in the laboratory. However, those who wish to use commercially available silver enhancement kits can continue to do so. All LM Grade colloidal gold-protein complexes are freeze-dried in buffer with stabilizers and a preservative. After reconstitution, they may be frozen in aliquots for extended storage.

This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
Jackson ImmunoResearch Laboratories, Inc. has trademark rights to AffiniPure™.