"The anti-human polyclonal capture reagents are some of the most robust reagents I have used in my biophysical and biochemical studies so far. Overall, the anti-Fc reagents regardless of the host or capturing species provides a robust capturing ability with minimum cross-reactivity to other species." Full review.
Rupesh Nanjunda,Rating: 5.0
"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio UniversityRating: 5.0
Jackson ImmunoResearch AffiniPure™ Anti-Human secondary antibodies are affinity purified polyclonal antibodies with specificity for human immunoglobulins.
We offer Anti-Human secondary antibodies in a range of host species including Alpaca, Donkey, Goat, Mouse and Rabbit. JIR secondary antibodies are available as whole IgG and F(ab')2 fragment formats. Fab fragment formats are also available and can be an elegant component of multiple labeling and blocking protocols.
They are available with specificity for whole Ig, Fcγ and F(ab')2 domains, among other specificities.
Anti-Human secondary antibodies are an invaluable reagent in the development of high-throughput serological testing kits. JIR Anti-Human antibodies are manufactured to ISO 9001:2015 certification and are available in standard and bulk quantities for worldwide shipment.
JIR Anti-Human secondary antibodies are suitable for the detection of human immunoglobulin isotypes by immunoassay including ELISA, ELISPOT, Rapid test, Enzyme immunoassay (EIA), Luminscent immunoassay (LIA), Fluorescent immunoassay (FIA) and Lateral Flow.
Available with specificities to IgG, IgM or IgA as well as IgG+IgM and IgG+IgM+IgA. Anti-Human products are highly cross-adsorbed.
Host species options include Alpaca, Donkey, Goat, Mouse and Rabbit.
Biotin, HRP and fluorescent dye conjugates available.
Affinity-purified antibodies are isolated from antisera by immunoaffinity chromatography using target antigens coupled to agarose beads. A proprietary elution process is used to dissociate antibodies from the antigen. Unconjugated affinity-purified antibodies are supplied sterile filtered in phosphate buffer without stabilizers or preservatives. Conjugated affinity-purified antibodies are freeze-dried in phosphate buffer with stabilizers and sodium azide, with the exception of horseradish peroxidase conjugates, which do not contain a preservative.
Secondary antibodies against one species are likely to cross-react with other species unless they have been specifically adsorbed against the other species. Antibodies with "(min X ... Sr Prot)" in the description have been tested and/or adsorbed against IgG and/or serum proteins of those species indicated in the parentheses. They are recommended when the presence of immunoglobulins from other species may lead to interfering cross-reactivities.
“When labeling for more than one target with antibodies from closely related species, you really need to certain your secondary antibody is specific!”
Read on to find out how Cardiac researcher Elisabeth Ehler uses Jackson ImmunoResearch cross-adsorbed (min X) secondary antibodies to multiple label with primary antibodies derived from closely related host species – enabling her to differentiate cell types in primary cardiac cell cultures.
Primary cultures of neonatal rat cardiomyocytes stained with Cy3-AffiniPure Goat Anti-Mouse IgG (H+L) (115-165-146) and Cy2-AffiniPure Goat Anti-Rabbit IgG (H+L) (111-225-144) secondary antibodies together with DAPI counterstain. Image courtesy of Elisabeth Ehler, KCL’
Alexa Fluor® fluorescent dyes are widely recognized as superior fluorescent dyes, respected for their brightness and photostability. They are suitable for a variety of applications including confocal microscopy, flow cytometry, fluorescent Western blotting, and fluorescent ELISA. Alexa Fluor conjugated secondary antibodies can be used exclusively or in combination with other fluorescent probes to generate an optimal multiplex dye panel for your experimental variables.
JIR provides Alexa Fluor 488, 594, 647, 680, 790 conjugates.
FabuLight antibodies are Fab fragment secondary antibodies specific to the Fc region of IgG or IgM primary antibodies.
They are available conjugated with 9 different fluorophores and biotin, and enable labeling of primary antibodies prior to incubation with cells or tissue.
They provide a time saving alternative to sequential incubation flow cytometry and immunohistochemistry procedures, without compromising the active site of the primary antibody.
Possible uses of FabuLights also include labeling cell surface immunoglobulins without cross-linking and activating B cells, and labeling Fc chimeras (fusion proteins).
