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In an immunohistochemistry or immunofluorescence experiment, proteins of interest can be visualized using fluorophore or enzyme-labeled antibodies. When viewed under the microscope the signal generated is specific to the protein of interest.
Watch this video to learn how to avoid background from endogenous immunoglobulins when labeling tissue of the same species as the primary antibodies (mouse primary on mouse tissue) with JIR Fab Fragment blocking!