Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Secondary Antibodies

For VHH Discovery

Anti-Alpaca IgG, Subclass and VHH domain secondaries
  • Ideally time PBMC harvest
  • Enhance detection of VHH antibodies
  • Test for VHH expression and binding activity

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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Biotin Conjugated Secondary Antibodies

Biotin-SP (long-spacer) Conjugated Secondary Antibodies

Biotin is a small molecule which non-covalently binds to avidin and streptavidin with very high affinity. The affinity of the interaction makes biotin an excellent conjugate for detection when used in immunohistochemistry techniques. Biotin conjugates can be employed in signal enhancement techniques.

Biotin-SP™ is our trade name for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin.

Biotinylated antibodies require additional reagents for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.

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Mouse Anti-Biotin Conjugates

Signal Enhancement with Biotinylated Secondary Antibodies

Signal Enhancement with Biotinylated Secondaries Labeled streptavidin-biotin (LSAB) method for signal amplification.
A. Label antigen with primary antibody.
B. Incubate with biotinylated secondary antibody
C. Incubate with conjugated streptavidin.

IHC with Biotinylated Secondaries Anti-GFP immunohistochemical staining using Biotin-SP-conjugated Donkey Anti-Rabbit IgG (H+L) secondary antibody followed by Peroxidase-conjugated Streptavidin: (A) At 4 weeks post-transplantation, no GFP signal could be detected in the in vivo specimens. (B) GFP expressing cells were visualized by brown staining in the positive control; signal was visualized using the chromogenic substrate 3,3’ diaminobenzidine (DAB) substrate. Nuclei were counter-stained with hematoxylin.