Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates
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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Brilliant Violet™ 421

Brilliant Violet™ 421 Technical Information

Brilliant Violet 421™ (BV421™) has an excitation peak at 407 nm with maximal emission at 421 nm. Brilliant Violet polymer chains can be considered as a collection of optical segments, each with the ability to absorb light and emit fluorescence signal. This results in conjugates that have a bright fluorescence signal for exceptional resolution and sensitivity. BV dyes are suitable for use with aqueous media.

BV421 is available conjugated to secondary antibodies suitable for multiple labeling

Excitation Peak
Emission Peak
Extinction Co-efficient
M -1 cm-1
Quantum Yield
(ɸ F)
Brilliant Violet™ 421 407 421 2587000 0.91

Brilliant Violet 421™ Conjugated Antibodies

Control Reagents Product Code
Brilliant Violet 421™ ChromPure Donkey IgG, whole molecule 017-670-003
Brilliant Violet 421™ ChromPure Goat IgG, whole molecule 005-670-003

Spectra Viewer

Use the spectra viewer below to compare BV421 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.

  Show Excitation and Emission Spectra  

  • Brilliant Violet™ is a trademark of Sirigen Inc., a Becton, Dickinson and Company affiliate.