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"We only use secondary antibodies from Jackson ImmunoResearch whenever possible! Customer service is also excellent and the price point for their products is very competitive."
Elizabeth Soberg, University of Washington
BD Brilliant™ Violet 421 (BV421) has an Excitation peak at 407 nm with maximal emission at 421 nm. Brilliant Violet polymer chains can be considered as a collection of optical segments, each with the ability to absorb light and emit fluorescence signal. This results in materials that have a bright fluorescence signal for better resolution and sensitivity.
5-color imaging is possible with BV421, in combination with BV480, Alexa Fluor® 488, Rhodamine-Red™-X, and Alexa Fluor® 647. Switching the nuclear stain from DAPI (emission in the blue region) to DRAQ5™ (which has red emission) frees the violet-blue region of the spectrum to accommodate the two Brilliant Violet dyes. DRAQ5's excitation and emission profiles overlap those of Alexa Fluor 647.
Excitation and emission spectra of Brilliant Violet™421-conjugated affinity-purified secondary antibodies and purified proteins. Peak heights have been normalized, spectra were obtained with an M-Series spectrophotometer system from Photon Technology International, Inc. Values are approximate, actual values may vary depending on the spectrofluorometer used in each laboratory.
M -1 cm-1
|Brilliant Violet™ 421||407||421||2587000||0.91|
Brilliant Violet™ is a trademark of Sirigen Inc., a Becton, Dickinson and Company affiliate.