Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Secondary Antibodies

For VHH Discovery

Anti-Alpaca IgG, Subclass and VHH domain secondaries
  • Ideally time PBMC harvest
  • Enhance detection of VHH antibodies
  • Test for VHH expression and binding activity

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Brilliant Violet™ 421

Brilliant Violet™ 421 Technical Information

BD Brilliant™ Violet 421 (BV421) has an Excitation peak at 407 nm with maximal emission at 421 nm. Brilliant Violet polymer chains can be considered as a collection of optical segments, each with the ability to absorb light and emit fluorescence signal. This results in materials that have a bright fluorescence signal for better resolution and sensitivity.

5-color imaging is possible with BV421, in combination with BV480, Alexa Fluor® 488, Rhodamine-Red™-X, and Alexa Fluor® 647. Switching the nuclear stain from DAPI (emission in the blue region) to DRAQ5™ (which has red emission) frees the violet-blue region of the spectrum to accommodate the two Brilliant Violet dyes. DRAQ5's excitation and emission profiles overlap those of Alexa Fluor 647.

Read More About Brilliant Violet™ Secondary Antibody Conjugates



Excitation Peak
(nm)
Emission Peak
(nm)
Extinction Co-efficient
M -1 cm-1
Quantum Yield
F)
Brilliant Violet™ 421 407 421 2587000 0.91

Brilliant Violet™ is a trademark of Sirigen Inc., a Becton, Dickinson and Company affiliate.

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