Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates
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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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DyLight 405

DyLight™ 405 Technical Information

DyLight™ 405 - conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use requires instruments equipped with a 405 nm laser and a 420 nm emission filter.

The combination of DyLight 405, Alexa Fluor® 488, Rhodamine Red™-X, and Alexa Fluor® 647 provides for maximum color separation, good photostability and high sensitivity.

DyLight 405 is available conjugated to:

Excitation Peak (nm) Emission Peak (nm)
DyLight 405 400 421

DyLight 405 Conjugated Antibodies


Control Reagents Product Code
DyLight™ 405-conjugated ChromPure Donkey IgG, whole molecule 017-470-003
DyLight™ 405-conjugated ChromPure Goat IgG, whole molecule 005-470-003
DyLight™ 405-conjugated ChromPure Human IgG, whole molecule 009-470-003
DyLight™ 405-conjugated ChromPure Mouse IgG, whole molecule 015-470-003
DyLight™ 405-conjugated ChromPure Rabbit IgG, whole molecule 011-470-003
DyLight™ 405-conjugated ChromPure Rat IgG, whole molecule 012-470-003
DyLight™ 405-conjugated ChromPure Sheep IgG, whole molecule 013-470-003
DyLight™ 405-conjugated ChromPure Bovine IgG, whole molecule 001-470-003
Streptavidin Product Code
DyLight™ 405-conjugated Streptavidin 016-470-084

Spectra Viewer

Use the spectra viewer below to compare DyLight 405 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.


  • DyLight™ is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
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