"Without question, I can always turn to Jackson ImmunoResearch secondary antibodies for ECL detection of any primary antibody! They produce great signal at low dilutions, last for years even through bad laboratory practice like repeatedly using the same tube every time, and come in a variety of species flavors."
W Thompson, OSHU
PerCP is a fluorescent peridinin-chlorophyll protein complex isolated from dinoflagellates. We offer the form found in Dinophyceae sp. with a molecular weight of about 35.5 kDa. It has a broad spectrum of excitation with a main peak at 472 nm, and a long Stokes shift to an emission peak at 677 nm (Figure 1).
PerCP conjugates of highly adsorbed secondary antibodies are offered to label unconjugated primary antibodies, and PerCP-streptavidin is offered to label biotinylated primary or secondary antibodies (Figures 2 and 3). Two practical labeling protocols are possible with the products.
Compared with a single-step PerCP-conjugated primary antibody (Figure 2), about the same level of fluorescence is obtained with a two-step procedure using a biotinylated primary antibody and PerCP-conjugated streptavidin. A consistent, slightly higher signal is achieved by using an unconjugated primary antibody and PerCP-conjugated secondary antibody. Although three-step procedures are usually undesirable for flow cytometry, a somewhat greater amplification may be obtained with unconjugated primary antibody, biotinylated secondary antibody, and PerCP-conjugated streptavidin (Figure 3).
PerCP conjugates may be used alone or with Alexa Fluor® 488 (or FITC) and R-PE for one- to three-color analyses with a single-laser flow cytometer equipped with an argon laser emiting at 488 nm. Up to four-color analyses with low compensation are easily achieved by adding APC-conjugated antibodies with 633 or 635 nm excitation provided by a dual-laser flow cytometer (Figure 4).