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"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."Janet Duerr, Ohio University
R-PE (R-Phycoerythrin) is a 240 kDa light-harvesting phycobiliprotein found in red macrophytic algae (seaweed). After phycobiliproteins are conjugated to secondary antibodies, there is little fluorescence quenching, which results in conjugates of high specific fluorescence compared with conventional fluorophore-antibody conjugates. R-PE can be excited by light over a wide range of the visible spectrum, is highly water soluble, has a relatively low isoelectric point, and lacks potentially sticky carbohydrates. The relatively high molecular weight of R-PE may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.
R-PE is available conjugated to:
|Excitation Peak (nm)||Emission Peak (nm)|
|R-Phycoerythrin R-PE||many, 488||580|
Use the spectra viewer below to compare R-PE with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.