Attention! We have implimented a new version of our Product Filter and if you have visited our site recently, you may need to clear your browser's cache to view it. Depending on your operating system all you need to do is press the following key combination: Windows: ctrl + F5, Mac/Apple: command + R or Linux: F5.
Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

Find By Code Number

Product Filter

Spotlight on...

Super Resolution Microscopy

Secondary antibodies conjugated with Alexa Fluor® 488 / 594 / 647 / 790, FITC, Cy3, and Cy5 for STED and STORM.

More info

Rhodamine Red-X, RRX

Rhodamine Red™-X, RRX Technical Information

RRX (Rhodamine Red-X) conjugates have a peak of excitation at 570 nm and a peak of emission at 590 nm. Although TRITC has been used traditionally with FITC for double labeling, better color separation is achieved by using RRX or Alexa Fluor® 594. Rhodamine Red-X is particularly useful for 3- and 4-color labeling with DyLight 405, Alexa Fluor® 488, and Alexa Fluor® 647 by using a confocal microscope equipped with a 405 nm laser and a krypton/argon laser. Fluorescence from RRX lies about midway between that of Alexa Fluor® 488 and Alexa Fluor® 647, and it shows little overlap with either dye. The krypton-argon laser emits lines at 488 nm, 568 nm, and 647 nm, which are optimal for exciting Alexa Fluor® 488, RRX, and Alexa Fluor® 647, respectively. By adding a 405 nm laser and a 420 nm emission filter, 4-color labeling is possible using DyLight 405-conjugated secondary antibodies from JIR. The separation between all four dyes is perfect for 4-color labeling, and all four dyes are very bright.

Rhodamine Red-X has good color separation from DyLight 405, and Alexa Fluor® 488, and Alexa Fluor® 647 for effective 4-color imaging.

Excitation and emission spectra of RRX-conjugated affinity-purified secondary antibodies, streptavidin, and purified proteins. Peak heights have been normalized, spectra were obtained with an M-Series spectrophotometer system from Photon Technology International, Inc. Values are approximate, actual values may vary depending on the spectrofluorometer used in each laboratory.

Emission spectra for optimal 4-color imaging using DyLight 405 (blue), Alexa Fluor® 488 (green), Rhodamine Red-X (red), and Alexa Fluor® 647 (purple).

Excitation Peak Emission Peak (nm)
Rhodamine Red-X, RRX 570 590

Rhodamine Red™-X is a trademark of Molecular Probes, Inc.