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"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."Janet Duerr, Ohio University
RRX (Rhodamine Red™-X) conjugates have peak excitation at 570 nm and peak emission at 590 nm. Rhodamine Red™-X is particularly useful for 3- and 4-color labeling with DyLight™ 405, Alexa Fluor® 488 and Alexa Fluor® 647 using a microscope equipped with an appropriate excitation source. In 3-color labeling, fluorescence from RRX lies about midway between that of Alexa Fluor® 488 and Alexa Fluor® 647, and it shows little overlap with either dye. Four-color labeling can be achieved with the addition of DyLight 405, whose emission can be separated from that of Alexa Fluor® 488.
RRX is available conjugated to:
|Excitation Peak||Emission Peak (nm)|
|Rhodamine Red-X, RRX||570||590|
Use the spectra viewer below to compare RRX with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.
Rhodamine Red™-X is a trademark of Molecular Probes, Inc.