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"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio University
Whole IgG antibodies are isolated as intact molecules from antisera by affinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds (Figure 1), and therefore are divalent. The average molecular weight is reported to be about 160 kD. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Browse our comprehensive selection of whole IgG secondary antibodies using link to the table below.
Note that these groups may include antibodies or conjugates not included in the main table of whole IgG secondary antibodies.
For flow cytometry we offer three fluorescent proteins (R-PE, APC, and PerCP) conjugated to many highly adsorbed whole IgG and F(ab') 2 fragment secondary antibodies. Also shown in this table are the same highly adsorbed antibodies conjugated to Biotin-SP and fluorescent dyes appropriate for flow cytometry (Alexa Fluor® 488, FITC, and Alexa Fluor® 647). Note that many antibodies listed elsewhere in tables of Whole IgG and F(ab')2 fragments also can be used for flow cytometry.
These antibodies have been extensively adsorbed against other mouse IgG subclasses. They are intended for distinguishing between two or more different subclasses of mouse IgG in multiple-labeling experiments, or for mouse IgG subclass determination.
A 50 kDa protein may be detected on blots without interference from the precipitated IgG in the same band, by incubation with its unlabeled primary antibody followed by labeled anti-IgG, Light Chain Specific secondary antibody.
Antibodies conjugated with far-red- and Infrared-emitting dyes are more sensitive than those with dyes emitting visible light due to low fluorescence quenching of the conjugates, high extinction coefficients of the dyes, and low background autofluorescence.
Cyanine dyes are better able to withstand the harsh dehydration and embedding conditions required for mounting sections in non-polar plastic media.
IgG fraction Monoclonal Mouse Anti-Biotin, Anti-Fluorescein, and Anti-Digoxin may be used either as direct conjugates, or for more sensitivity, they can be used unconjugated followed by a conjugated anti-mouse IgG (H+L) for signal enhancement.
Affinity-purified anti horseradish peroxidase (HRP) may be used to detect HRP or to enhance signal by binding to HRP-conjugated molecules.
Colloidal gold reagents are available either for transmission and scanning electron microscopy (EM Grade) or for light microscopy and immunoblotting (LM Grade).
We also have a limited inventory of DyLight™ 488 / 549 / 594 / 647, Cy™2, Cy™5, and Texas Red® conjugated secondary antibodies.