Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates
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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Whole IgG Secondary Antibodies

Whole IgG Affinity-Purified Secondary Antibodies

Whole IgG antibodies are isolated as intact molecules from antisera by affinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds (Figure 1), and therefore are divalent. The average molecular weight is reported to be about 160 kD. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Whole IgG
Figure 1. Schematic representation of a whole IgG molecule.

Browse our comprehensive selection of whole IgG secondary antibodies using link to the table below.

Whole IgG Secondary Antibodies For Specific Purposes

Note that these groups may include antibodies or conjugates not included in the main table of whole IgG secondary antibodies.

R-PE, APC, and PerCP, Conjugates for Flow Cytometry

For flow cytometry we offer three fluorescent proteins (R-PE, APC, and PerCP) conjugated to many highly adsorbed whole IgG and F(ab')2 fragment secondary antibodies. Also shown in this table are the same highly adsorbed antibodies conjugated to Biotin-SP and fluorescent dyes appropriate for flow cytometry (Alexa Fluor® 488, FITC, and Alexa Fluor® 647). Note that many antibodies listed elsewhere in tables of Whole IgG and F(ab')2 fragments also can be used for flow cytometry.

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Antibodies for Signal Enhancement

IgG fraction Monoclonal Mouse Anti-Biotin, Anti-Fluorescein, and Anti-Digoxin may be used either as direct conjugates, or for more sensitivity, they can be used unconjugated followed by a conjugated anti-mouse IgG (H+L) for signal enhancement.

Affinity-purified anti horseradish peroxidase (HRP) may be used to detect HRP or to enhance signal by binding to HRP-conjugated molecules.

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Anti‑Light Chain-Specific Antibodies for Western Blotting after IP

A 50 kDa protein may be detected on blots without interference from the precipitated IgG in the same band, by incubation with its unlabeled primary antibody followed by labeled anti-IgG, Light Chain Specific secondary antibody.

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Alexa Fluor® 680 and Alexa Fluor® 790 for High Sensitivity

Antibodies conjugated with far-red- and Infrared-emitting dyes are more sensitive than those with dyes emitting visible light due to low fluorescence quenching of the conjugates, high extinction coefficients of the dyes, and low background autofluorescence.

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Cy2, Cy3, and Cy5 for Permanent Mounts

Cyanine dyes are better able to withstand the harsh dehydration and embedding conditions required for mounting sections in non-polar plastic media.

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Anti-Mouse IgG Subclass Specific Antibodies

These antibodies have been extensively adsorbed against other mouse IgG subclasses. They are intended for distinguishing between two or more different subclasses of mouse IgG in multiple-labeling experiments, or for mouse IgG subclass determination.

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LM and EM Grade Colloidal Gold - Antibody Complexes

Colloidal gold reagents are available either for transmission and scanning electron microscopy (EM Grade) or for light microscopy and immunoblotting (LM Grade).

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Anti-Alpaca Secondary Antibodies

Jackson ImmunoResearch offers a range of secondary antibodies utilizing the properties of camelid immunoglobulins, for detection in assays and to expedite VHH development.

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Anti-Human Secondary Antibodies

Anti-Human secondary antibodies are an invaluable reagent in the development of high-throughput serological testing kits.

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Anti-Chicken Secondary Antibodies

Anti‑Chicken antibodies are useful as a component of Lateral flow immunoassays.

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We also have a limited inventory of DyLight™ 488 / 549 / 594 / 647, Cy™2, Cy™5, and Texas Red® conjugated secondary antibodies.