Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Secondary Antibodies

For VHH Discovery

Anti-Alpaca IgG, Subclass and VHH domain secondaries
  • Ideally time PBMC harvest
  • Enhance detection of VHH antibodies
  • Test for VHH expression and binding activity

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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Alpaca Secondary Antibodies

Introducing NEW Alpaca Secondary Antibodies

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Immunofluorescence

Jackson ImmunoResearch continues to expand our comprehensive range of high quality secondary antibodies for the life science community.

The latest addition to our catalog is a new host species - Alpaca!

This presents a new species option when goat or donkey secondaries may not be preferable, for example in a multiple labeling assay which has potential unwanted cross-reactions. Secondary antibodies produced in alpaca exhibit inherent minimal cross-reactivity with camelid-derived antibodies (e.g. VHH antibodies, single domain antibodies, nanobodies).

Alpacas are part of the camelid family, along with llamas and camels. JIR’s affinity purified polyclonal antibodies produced by alpacas are predominantly IgG1 (the conventional subclass featuring both heavy and light chains) and have binding properties that are similar to those derived from the more familiar host species. These antibodies also include the heavy chain only subclasses IgG2 and IgG3 (Learn about IgG2 and 3  here).

JIR’s current offerings for alpaca secondary antibodies are prepared to ensure minimal cross-reactivity with bovine serum proteins to avoid background from BSA blocking steps and cell culture components. Further cross-adsorptions enable multiple labeling with the other available target species as shown below.

See an explanation of requirements for multiple labeling protocols here

Alpaca Secondary antibodies available

Conjugate Fill Size Alpaca Anti-Human IgG (H+L)
(min X Bov, Ms, Rb Sr Prot)
Alpaca Anti-Mouse IgG (H+L)
(min X Bov, Ms, Rb Sr Prot)
Alpaca Anti-Rabbit IgG (H+L)
(min X Bov, Ms, Rb Sr Prot)
Unconjugated 1mg 609-005-213 615-005-214 611-005-215
Horseradish Peroxidase 1ml 609-035-213 615-035-214 611-035-215
Alkaline Phosphatase 1ml 609-055-213 609-055-214 611-055-215
Biotin-SP™ 1ml 609-065-213 609-065-214 611-065-215
DyLight™ 405 1mg 609-475-213 609-475-214 611-475-215
AMCA 1mg 609-155-213 609-155-214 611-155-215
Cy™ 2 1mg 609-225-213 609-225-214 611-225-215
Alexa Fluor® 488 1mg 609-545-213 609-545-214 611-545-215
Fluorescein (FITC) 1mg 609-095-213 609-095-214 611-095-215
Cy™ 3 1mg 609-165-213 609-165-214 611-165-215
Alexa Fluor® 594 1mg 609-585-213 609-585-214 611-585-215
Rhodamine Red-X 1mg 609-295-213 609-295-214 611-295-215
Alexa Fluor® 647 1mg 609-605-213 609-605-214 611-605-215
Cy™ 5 1mg 609-175-213 609-175-214 611-175-215

View Alpaca Secondary Antibodies

Normal Alpaca Serum is available for blocking.

Normal Alpaca Serum Fill Size
028-000-001 2.0 ml
028-000-121 10.0 ml

See blocking guide

† Nanobody and Nanobodies are registered trademarks of Ablynx N.V.

DyLight™ fluorescent dyes is a trademark of Thermo Fisher Scientific.

Cy™ and CyDye® are registered trademarks of GE Healthcare.

Rhodamine Red™-X is a trademark of Invitrogen.

Alexa Fluor® is a trademark of Life Technologies Corp.

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