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"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio University
Cy™5 conjugates are excited maximally at 650 nm and fluoresce maximally at 670 nm. They can be excited to about 98% of maximum with a krypton/argon laser (647 nm line) or to about 63% of maximum with a helium/neon laser (633 nm line). Cy™5 can be used with a variety of other fluorophores for multiple labeling due to a wide separation of its emission from that of shorter wavelength-emitting fluorophores. When specimens are mounted in non-polar, plastic mounting media, Cy™5 is brighter than other far-red-fluorescing dyes, including Alexa Fluor® 647.
Cy5 is available conjugated to:
|Excitation Peak (nm)||Emission Peak (nm)|
Use the spectra viewer below to compare Cy5 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.
Cy™ is a trademark of GE Healthcare Bio-Sciences Ltd.