Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Alexa Fluor Secondary Antibodies

Alexa Fluor® Secondary Antibodies

Alexa Fluor® fluorescent dyes are widely recognized as superior fluorescent dyes available for conjugation. They are highly water soluble and remain fluorescent from pH 4 to pH 10. The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per mole of antibody. These parameters have been researched for each dye conjugate to optimize the level of antibody detection and minimize background.


Alexa Fluor® Secondary Antibodies are Available in a Range of Formats

Whole IgG Alexa Fluor® Secondary Antibodies

Whole IgG antibodies are isolated as intact molecules from antisera by affinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds (Figure 1), and therefore are divalent. The average molecular weight is reported to be about 160 kD. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Alexa Fluor 488 IF Image
GFAPalpha DAPI primary astrocyte culture 1. Primary mixed glial culture derived from P0-P3 mouse pups, stained for GFAP C-term (Santa Cruz sc-6170) and donkey-anti-goat Alexa Fluor® 488 (Jackson ImmunoResearch 705-546-147). Authors: Carlyn Martina-Mamber, Elly M. Hol.

F(ab')2 Alexa Fluor® Secondary Antibodies

F(ab')2 fragments of antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving intact some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds, and therefore are divalent (Figure 1). The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of antibodies to live cells with Fc receptors or to Protein A or Protein G.

Alexa Fluor® Fab fragments

Fab fragments are the antibody binding regions of an antibody. An IgG immunoglobulin contains two, comprising the arms of the classic Y shape. The Fab fragments can be isolated from the Fc portion, and from each other, by papain digestion. This results in a monovalent (one binding site) antibody. They are useful for:

  • Blocking Endogenous Immunoglobulins to reduce background staining
  • Double Labeling Primary Antibodies from the Same Host Species

FabuLight™ - Label Primary Antibodies In Solution Without Compromising Activity

FabuLight antibodies are Fab fragment secondary antibodies specific to the Fc region of IgG or IgM primary antibodies. They are available conjugated with 9 different fluorophores and biotin, and enable labeling of primary antibodies prior to incubation with cells or tissue. This provides a time saving alternative to sequential incubation flow cytometry and immunohistochemistry procedures, without compromising the active site of the primary antibody.



Alexa Fluor® Excitation Peak Emission Peak (nm)
Alexa Fluor® 488 493 519
Alexa Fluor® 594 591 614
Alexa Fluor® 647 651 667
Alexa Fluor® 680 684 702
Alexa Fluor® 790 792 803

Table 1. Approximate peak wavelengths of excitation and emission for Alexa Fluor®-conjugated, affinity-purified secondary antibodies offered by Jackson ImmunoResearch in order by increasing peak of emission. Approximate values are given for purposes of comparing one fluorophore with another. Actual values may vary depending on the spectrofluorometer used in each laboratory.

Alexa Fluor® fluorescent dyes are a trademark of Life Technologies Corp. They are provided under an agreement between Life Technologies Corp and Jackson ImmunoResearch Laboratories, Inc and the manufacture, use, sale, or import of these dyes is sold pursuant to a license from Life Technologies Corp for use of its fluorescent dye technology. The sale of these dyes or products containing these dyes is expressly conditioned on the buyer not using them (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic, or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corp, 5791 Van Allen Way, Carlsbad, CA 92008 or outlicensing@lifetech.com.


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