Fab Fragments for Blocking and Double
Labeling of Primary Antibodies from the
Same Host Species
Example E.Detection of one unlabeled and one or more labeled primary antibodies without the use of Fab fragments
- Incubate with the first primary antibody, in this example Rabbit Anti-Antigen X. Wash.
- Incubate with Probe I-conjugated secondary antibody, in this example Alexa Fluor® 488-Goat Anti-Rabbit IgG
- Incubate with normal serum from the host species of the primary antibody, in this example normal rabbit
- Incubate with Probe II-conjugated primary antibody, in this example Rhodamine Red-X-Rabbit Anti-Antigen Y.
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