Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Multiple Labeling

With BV421™ & BV480™

  • Combine with AF488, RR-X, and AF647 for 5-color imaging
  • Compatible with commonly used filter sets
  • Switch nuclear stain from DAPI to DRAQ5 for additional labeling options

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Whole IgG Affinity-Purified Secondary Antibodies

"We only use secondary antibodies from Jackson ImmunoResearch whenever possible! Customer service is also excellent and the price point for their products is very competitive."

Elizabeth Soberg, University of Washington

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Technical Service e-mail
tech@jacksonimmuno.com

Fab Fragments for Blocking and Double Labeling of Primary Antibodies from the Same Host Species

Example E.Detection of one unlabeled and one or more labeled primary antibodies without the use of Fab fragments

  1. Incubate with the first primary antibody, in this example Rabbit Anti-Antigen X. Wash.

  2. Incubate with Probe I-conjugated secondary antibody, in this example Alexa Fluor® 488-Goat Anti-Rabbit IgG (H+L). Wash.

  3. Incubate with normal serum from the host species of the primary antibody, in this example normal rabbit serum. Wash.

  4. Incubate with Probe II-conjugated primary antibody, in this example Rhodamine Red-X-Rabbit Anti-Antigen Y. Wash.

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