Fab Fragments for Blocking and Double
Labeling of Primary Antibodies from the
Same Host Species
Example D. Use of unconjugated Fab fragments for detection of one unlabeled and one or more labeled primary antibodies
- Incubate with Probe I conjugated to the first primary antibody, in this example
Alexa Fluor® 488-Rabbit Anti-Antigen X. Wash.
- Incubate with an excess of unconjugated Fab Goat Anti-Rabbit IgG (H+L). Wash.
- Incubate with the unconjugated primary antibody, in this example Rabbit Anti-Antigen Y. Wash.
- Incubate with Probe II-conjugated secondary antibody, in this example Rhodamine Red-X-Goat Anti-Rabbit IgG (H+L). Wash.
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