Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Fluorescent Dyes

Brilliant Violet™
421 & 480

  • Combine with AF488, RR-X, & AF647 for 5-color imaging
  • Compatible with commonly used filter sets
  • Switch nuclear stain from DAPI to DRAQ5™ for additional labeling options

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Whole IgG Affinity-Purified Secondary Antibodies

"Without question, I can always turn to Jackson ImmunoResearch secondary antibodies for ECL detection of any primary antibody! They produce great signal at low dilutions."

W Thompson, OSHU

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Technical Service e-mail
tech@jacksonimmuno.com

Fab Fragments for Blocking and Double Labeling of Primary Antibodies from the Same Host Species

Example D. Use of unconjugated Fab fragments for detection of one unlabeled and one or more labeled primary antibodies

  1. Incubate with Probe I conjugated to the first primary antibody, in this example Alexa Fluor® 488-Rabbit Anti-Antigen X. Wash.

  2. Incubate with an excess of unconjugated Fab Goat Anti-Rabbit IgG (H+L). Wash.

  3. Incubate with the unconjugated primary antibody, in this example Rabbit Anti-Antigen Y. Wash.

  4. Incubate with Probe II-conjugated secondary antibody, in this example Rhodamine Red-X-Goat Anti-Rabbit IgG (H+L). Wash.

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