Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Multiple Labeling

With BV421™ & BV480™

  • Combine with AF488, RR-X, and AF647 for 5-color imaging
  • Compatible with commonly used filter sets
  • Switch nuclear stain from DAPI to DRAQ5 for additional labeling options

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Whole IgG Affinity-Purified Secondary Antibodies

"We only use secondary antibodies from Jackson ImmunoResearch whenever possible! Customer service is also excellent and the price point for their products is very competitive."

Elizabeth Soberg, University of Washington

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Fab Fragments for Blocking and Double Labeling of Primary Antibodies from the Same Host Species

Example D. Use of unconjugated Fab fragments for detection of one unlabeled and one or more labeled primary antibodies

  1. Incubate with Probe I conjugated to the first primary antibody, in this example Alexa Fluor® 488-Rabbit Anti-Antigen X. Wash.

  2. Incubate with an excess of unconjugated Fab Goat Anti-Rabbit IgG (H+L). Wash.

  3. Incubate with the unconjugated primary antibody, in this example Rabbit Anti-Antigen Y. Wash.

  4. Incubate with Probe II-conjugated secondary antibody, in this example Rhodamine Red-X-Goat Anti-Rabbit IgG (H+L). Wash.

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