- Technical Support
- Custom Services
"We only use secondary antibodies from Jackson ImmunoResearch whenever possible! Customer service is also excellent and the price point for their products is very competitive."
Elizabeth Soberg, University of Washington
Technical Service e-mail
Caution: This protocol may be subject to the following problems:
(1) Example A may require a high concentration of conjugated Fab to achieve effective blocking of the first primary antibody. If this results in unacceptable background, try a lower concentration of the conjugated Fab, followed by further blocking with unconjugated Fab.
(2) If small aggregates of conjugated Fab are bound to the first primary antibody, they may act as divalent or polyvalent antibodies and capture some of the second primary antibodies, which would result in overlapping detection of antigens.