Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates
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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Fluorescein, FITC/DTAF

FITC Technical Information

FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all JIR antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm. Although less bright than other green-fluorescing dyes, FITC is a widely used fluorophore for applications that don’t require exquisite sensitivity. The major disadvantage of fluorescein is its rapid photobleaching (fading), which can be mitigated by the use of an anti-fading agent in the mounting medium. An alternative choice for many applications involving FITC is Alexa Fluor® 488 because it is brighter and more photostable.

DTAF (Dichlorotriazinylamino fluorescein) is another form of fluorescein, with excitation and emission peaks identical to those of FITC. Jackson ImmunoResearch uses DTAF (instead of FITC) for conjugation with streptavidin, since fluorescence from FITC is greatly quenched after conjugation with streptavidin. This phenomenon is unique to streptavidin, and is not observed with antibodies.

FITC is available conjugated to:

Excitation Peak (nm) Emission Peak (nm)
Fluorescein, FITC/DTAF 493 519

FITC Conjugated Antibodies

Control Reagents Product Code
ChromPure Bovine IgG, whole molecule 001-090-003
ChromPure Chicken IgY (IgG), whole molecule 003-090-003
ChromPure Donkey IgG, whole molecule 017-090-003
ChromPure Donkey IgG, F(ab')2 fragment 017-090-006
ChromPure Goat IgG, whole molecule 005-090-003
ChromPure Goat IgG, F(ab')2 fragment 005-090-006
ChromPure Syrian Hamster IgG, whole molecule 007-090-003
ChromPure Human IgG, whole molecule 009-090-003
ChromPure Human IgG, Fc fragment 009-090-008
ChromPure Human IgG, F(ab')2 fragment 009-090-006
ChromPure Human IgG, Fab fragment 009-090-007
ChromPure Human IgM (myeloma), whole molecule 009-090-012
ChromPure Human Serum IgA, whole molecule 009-090-011
ChromPure Human Albumin 009-090-051
ChromPure Human Transferrin 009-090-050
ChromPure Mouse IgG, whole molecule 015-090-003
ChromPure Mouse IgG, Fc fragment 015-090-008
ChromPure Mouse IgG, F(ab')2 fragment 015-090-006
ChromPure Mouse IgG, Fab fragment 015-090-007
ChromPure Mouse Transferrin 015-090-050
ChromPure Rabbit IgG, whole molecule 011-090-003
ChromPure Rat IgG, whole molecule 012-090-003
ChromPure Rat Transferrin 012-090-050
ChromPure Sheep IgG, whole molecule 013-090-003
Streptavidin Product Code
Fluorescein (DTAF) Streptavidin 016-010-084

Spectra Viewer

Use the spectra viewer below to compare FITC with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.

  Show Excitation and Emission Spectra