Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates
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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Anti-Mouse Secondary Antibodies

Anti-Mouse Secondary Antibodies

Jackson ImmunoResearch AffiniPure™ Anti-Mouse secondary antibodies are affinity purified polyclonal antibodies with specificity for mouse immunoglobulins.

We offer Anti-Mouse secondary antibodies in a range of host species including Alpaca, Donkey, Goat, Rabbit, Rat and Sheep. JIR secondary antibodies are available as whole IgG and F(ab')2 fragment formats. Fab fragment formats are also available and can be an elegant component of multiple blocking and labeling protocols.

They are available with with specificity for mouse IgG subclasses, light chains, Fcγ, F(ab')2 domains, and IgM µ chain, among other specificities.

Cultured mouse mammary gland tumour cells 4T1. Formalin fixed cells stained with anti alpha tubulin. Alpha tubulin visualised with Alexa Fluor® 488 Goat anti Mouse IgG₁. Nucleus counterstained with DAPI.
Cultured mouse mammary gland tumour cells 4T1 grown on glass coverslips. Formalin fixed cells have been stained with anti alpha tubulin to reveal microtubules. The alpha tubulin was visualised with Alexa Fluor® 488 Goat anti Mouse IgG₁. Nucleus counterstained with DAPI.

Anti-Mouse Secondary Antibodies are available with the following conjugates:

  • Unconjugated
  • Reporter enzymes (Alkaline Phosphatase (AP) or Horseradish Peroxidase (HRP)
  • Biotinylated (biotin conjugates)
  • Fluorophores (Alexa Fluor®, Brilliant Violet™, Cyanine and protein dye conjugates)
  • Colloidal Gold (Immunogold)

Affinity-purified antibodies are isolated from antisera by immunoaffinity chromatography using target antigens coupled to agarose beads. A proprietary elution process is used to dissociate antibodies from the antigen. Unconjugated affinity-purified antibodies are supplied sterile filtered in phosphate buffer without stabilizers or preservatives. Conjugated affinity-purified antibodies are freeze-dried in phosphate buffer with stabilizers and sodium azide, with the exception of horseradish peroxidase conjugates, which do not contain a preservative.

Cross adsorbed secondary antibodies (min X ... Sr Prot)

Secondary antibodies against one species are likely to cross-react with other species unless they have been specifically adsorbed against the other species. Antibodies with "(min X ... Sr Prot)" in the description have been tested and/or adsorbed against IgG and/or serum proteins of those species indicated in the parentheses. They are recommended when the presence of immunoglobulins from other species may lead to interfering cross-reactivities.

Mouse Subclass-Specific Dot Blot
Dot blot showing the specificity of goat anti-mouse IgG, Fcγ subclass specific antibodies.

Distinguish between different mouse IgG subclasses

Jackson ImmunoResearch anti-mouse IgG, subclass specific antibodies offer specificity to the 5 individual mouse IgG subclasses. They are designed to distinguish between two or more different subclass of mouse IgG in multiple labeling experiments, or for mouse IgG subclass determination.

They have been cross adsorbed against human, bovine and rabbit serum proteins to minimize cross-reactivity with tissue immunoglobulins. They are available conjugated to Alexa Fluor® and Cyanine™ Fluorescent dyes, Biotin-SP™, and reporter molecules.

Anti-Mouse Secondary Antibodies

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