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"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio University
Alexa Fluor® 680 is a far-red-emitting dye with peak excitation at 684nm and peak emission at 702nm. Alexa Fluor® 790 is an infrared-emitting dye with peak excitation at 783nm and peak emission at 803nm (Figure 1). Antibodies conjugated with far-red- and Infrared-emitting dyes are more sensitive than those with dyes emitting visible light due to low fluorescence quenching of the conjugates, high extinction coefficients of the dyes, and low background autofluorescence. The increased brightness allows for a wider range of immunofluorescence detection and imaging modalities. Far-red and Infrared dye conjugates can be used for higher sensitivity Western blots, quantitative Western blots, in-gel Western blots, microWestern arrays, in-cell Western arrays, on-cell Western arrays, tissue section imaging, small animal whole body imaging, and other techniques that require the brightest dyes.
Jackson ImmunoResearch now offers the largest selection of Alexa Fluor® 680 and Alexa Fluor® 790 dyes conjugated with signal-enhancing primary antibodies, affinity-purified secondary antibodies, streptavidin, and purified IgG controls for single- and double-labeling Western blots (Figure 2) and other techniques requiring high sensitivity. The secondary antibodies are adsorbed to eliminate cross-reactions with others species and with other immunoglobulin classes for double labeling.
Both dyes can be used with LiCor Odyssey imaging systems and all of the high-sensitivity techniques listed above. We recommend that the antibodies be diluted at least 1:50,000 to 1:200,000 due to the high sensitivity of the conjugates. Click here to see our table of Alexa Fluor® 680 and Alexa Fluor® 790 dyes conjugated to signal-enhancing primary antibodies, affinity-purified secondary antibodies, streptavidin, and purified IgG controls.