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"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."Janet Duerr, Ohio University
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Fluorescent Western blotting allows multiplex detection without stripping and reprobing for analytes, and can be used for quantitative detection. Various fluorescent conjugates can be employed depending on the instrument setup. For optimal detection, Alexa Fluor® 680 and Alexa Fluor® 790 conjugates can be used to achieve high sensitivity Western blots.
Antibodies conjugated with far-red- and infrared-emitting dyes are suitable for a variety of techniques requiring the highest sensitivity. Alexa Fluor® 680 and 790 dyes are more sensitive than visible light-emitting dyes due to lower fluorescence quenching of the conjugates and higher extinction coefficients of the dyes. Low sample autofluorescence in this region of the spectrum results in lower background signal compared with other fluorophores.
Alexa Fluor® 680 and 790 conjugates can be used for high sensitivity Western blots, quantitative Western blots, in-gel Western blots, microWestern arrays, in-cell Western assays, on-cell Western assays, and other techniques that require the brightest dyes.
The increased brightness of far-red and infrared dye conjugates (Alexa Fluor® 680 and 790) allows for a wide range of immunofluorescence detection and imaging modalities.
Alexa Fluor® 680 and 790 secondary antibodies are adsorbed to minimize cross-reactions with others species and/or with other immunoglobulin classes, facilitating multiple labeling.
With modern flow cytometers now able to accommodate longer wavelengths, Alexa Fluor® 680 and 790 provide expanded choice for flow cytometry dye panels.
Jackson ImmunoResearch offers a comprehensive selection of Alexa Fluor® 680 and Alexa Fluor® 790 dyes conjugated to secondary antibodies, streptavidin, signal enhancing antibodies and purified Donkey, Goat and Mouse IgG controls.