Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Anti-Rabbit Secondary Antibodies

Anti-Rabbit Secondary Antibodies

Jackson ImmunoResearch AffiniPure™ Anti-Rabbit secondary antibodies are affinity purified polyclonal antibodies with specificity for rabbit immunoglobulins.

We offer Anti-Rabbit secondary antibodies in a range of host species including Alpaca, Donkey, Goat, Rabbit, Rat and Sheep. JIR secondary antibodies are available as whole IgG and F(ab')2 fragment formats. Fab fragment formats are also available and can be an elegant component of multiple blocking and labeling protocols.

They are available with with specificity for whole Ig, Fcγ, and F(ab')2 domains, among other specificities.

Skin sweat gland labeled with rabbit anti-PGP9.5 + donkey anti-rabbit Cy3, epithelial cells labeled with rat anti-CD44 + donkey anti-rat Cy3 & basement membranes labeled with goat anti-collagen IV + donkey anti-goat Cy5

Skin sweat gland nerve fibers labeled with rabbit anti-PGP9.5 + donkey anti-rabbit Cy™3 (711-165-152 green), secretory epithelial cells labeled with rat anti-CD44 + donkey anti-rat Cy3 (712-166-150 red) and basement membranes labeled with goat anti-collagen IV + donkey anti-goat Cy™5 (705-175-147 blue)

Anti-Rabbit Secondary Antibodies are available with the following conjugates:

  • Unconjugated
  • Reporter enzymes (Alkaline Phosphatase (AP) or Horseradish Peroxidase (HRP)
  • Biotinylated (biotin conjugates)
  • Fluorophores (Alexa Fluor®, Brilliant Violet™, Cyanine and protein dye conjugates)
  • Colloidal Gold (Immunogold)

Affinity-purified antibodies are isolated from antisera by immunoaffinity chromatography using target antigens coupled to agarose beads. A proprietary elution process is used to dissociate antibodies from the antigen. Unconjugated affinity-purified antibodies are supplied sterile filtered in phosphate buffer without stabilizers or preservatives. Conjugated affinity-purified antibodies are freeze-dried in phosphate buffer with stabilizers and sodium azide, with the exception of horseradish peroxidase conjugates, which do not contain a preservative.

Cross adsorbed secondary antibodies (min X ... Sr Prot)

Secondary antibodies against one species are likely to cross-react with other species unless they have been specifically adsorbed against the other species. Antibodies with "(min X ... Sr Prot)" in the description have been tested and/or adsorbed against IgG and/or serum proteins of those species indicated in the parentheses. They are recommended when the presence of immunoglobulins from other species may lead to interfering cross-reactivities.



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