Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates
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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Alexa Fluor® 594

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Alexa Fluor® 594 Technical Information

Alexa Fluor® 594 - conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. They are brighter, more photostable and more hydrophilic than Texas Red conjugates. Alexa Fluor® 594 conjugates are brighter than other red-fluorescing conjugates, and they provide better color separation from green-fluorescing dyes than Cy™3 and TRITC conjugates. They are the best choice for immunofluorescence detection in the deep-red region of the visible spectrum.

Alexa Fluor® 594 is available conjugated to:

Laser line (nm)
Common filter set
Excitation (nm)
Emission (nm)
Extinction co-efficient
Quantum Yield (Φ)
Molecular Weight (kDa)
Alexa Fluor® 594 561/594 591 618 1.4 0.66 618 Microscopy, Flow Cytometry, Fluorisa, Fluorescent Western Blotting
Dye Alexa Fluor® 594
Laser line (nm) 561/594
Common filter set
Excitation (nm) 591
Emission (nm) 618
Extinction co-efficient 1.4
Quantum Yield (Φ) 0.66
Molecular Weight (kDa) 618
Applications Microscopy
Flow Cytometry
Fluorescent Western Blotting

Alexa Fluor® 594 Conjugated Antibodies

Control Reagents Product Code
Alexa Fluor® 594-conjugated ChromPure Bovine IgG, whole molecule 001-580-003
Alexa Fluor® 594-conjugated ChromPure Chicken IgY (IgG), whole molecule 003-580-003
Alexa Fluor® 594-conjugated ChromPure Donkey IgG, whole molecule 017-580-003
Alexa Fluor® 594-conjugated ChromPure Goat IgG, whole molecule 005-580-003
Alexa Fluor® 594-conjugated ChromPure Human IgG, whole molecule 009-580-003
Alexa Fluor® 594-conjugated ChromPure Mouse IgG, whole molecule 015-580-003
Alexa Fluor® 594-conjugated ChromPure Rabbit IgG, whole molecule 011-580-003
Alexa Fluor® 594-conjugated ChromPure Rat IgG, whole molecule 012-580-003
Alexa Fluor® 594-conjugated ChromPure Sheep IgG, whole molecule 013-580-003
Streptavidin Product Code
Alexa Fluor® 594-conjugated Streptavidin 016-580-084

Spectra Viewer

Use the spectra viewer below to compare Alexa Fluor® 594 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.

  Show Excitation and Emission Spectra  

  • Alexa Fluor® fluorescent dyes is a trademark of Life Technologies Corp.