"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio UniversityRating: 5.0
Alexa Fluor® 594 - conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. They are brighter, more photostable and more hydrophilic than Texas Red conjugates. Alexa Fluor® 594 conjugates are brighter than other red-fluorescing conjugates, and they provide better color separation from green-fluorescing dyes than Cy™3 and TRITC conjugates. They are the best choice for immunofluorescence detection in the deep-red region of the visible spectrum.
Alexa Fluor® 594 is available conjugated to:
Dye |
Laser line (nm) |
Common filter set |
Excitation (nm) |
Emission (nm) |
Extinction co-efficient |
Quantum Yield (Φ) |
Molecular Weight (Da) |
Applications |
|---|---|---|---|---|---|---|---|---|
| Alexa Fluor® 594 | 561/594 | 591 | 618 | 1.4 | 0.66 | 618 | Microscopy, Flow Cytometry, Fluorisa, Fluorescent Western Blotting |
| Dye | Alexa Fluor® 594 |
|---|---|
| Laser line (nm) | 561/594 |
| Common filter set | |
| Excitation (nm) | 591 |
| Emission (nm) | 618 |
| Extinction co-efficient | 1.4 |
| Quantum Yield (Φ) | 0.66 |
| Molecular Weight (Da) | 618 |
| Applications | Microscopy |
| Flow Cytometry | |
| Fluorisa | |
| Fluorescent Western Blotting |
| Control Reagents | Product Code |
|---|---|
| Alexa Fluor® 594-conjugated ChromPure Bovine IgG, whole molecule | 001-580-003 |
| Alexa Fluor® 594-conjugated ChromPure Chicken IgY (IgG), whole molecule | 003-580-003 |
| Alexa Fluor® 594-conjugated ChromPure Donkey IgG, whole molecule | 017-580-003 |
| Alexa Fluor® 594-conjugated ChromPure Goat IgG, whole molecule | 005-580-003 |
| Alexa Fluor® 594-conjugated ChromPure Human IgG, whole molecule | 009-580-003 |
| Alexa Fluor® 594-conjugated ChromPure Mouse IgG, whole molecule | 015-580-003 |
| Alexa Fluor® 594-conjugated ChromPure Rabbit IgG, whole molecule | 011-580-003 |
| Alexa Fluor® 594-conjugated ChromPure Rat IgG, whole molecule | 012-580-003 |
| Alexa Fluor® 594-conjugated ChromPure Sheep IgG, whole molecule | 013-580-003 |
| Streptavidin | Product Code |
| Alexa Fluor® 594-conjugated Streptavidin | 016-580-084 |
Use the spectra viewer below to compare Alexa Fluor® 594 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.
