"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio UniversityRating: 5.0
Alexa Fluor® 680 conjugates are used for very sensitive Western blots, ELISAs and multiplexing arrays. Alexa Fluor® 680 conjugates are excited with a peak around 684 nm and fluoresce with a peak around 702 nm. They can be visualized with a LI-COR Odyssey® imager and other fluorescence detection systems, and they can be used for double labeling with Alexa Fluor® 790 conjugates.
Alexa Fluor® 680 is available conjugated to:
Dye |
Laser line (nm) |
Common filter set |
Excitation (nm) |
Emission (nm) |
Extinction co-efficient |
Quantum Yield (Φ) |
Molecular Weight (kDa) |
Applications |
|---|---|---|---|---|---|---|---|---|
| Alexa Fluor® 680 | 633 | 684 | 702 | 1.4 | N/A | 1150 | Microscopy, Flow Cytometry, Fluorisa, Fluorescent Western Blotting |
| Dye | Alexa Fluor® 680 |
|---|---|
| Laser line (nm) | 633 |
| Common filter set | |
| Excitation (nm) | 684 |
| Emission (nm) | 702 |
| Extinction co-efficient | 1.4 |
| Quantum Yield (Φ) | N/A |
| Molecular Weight (kDa) | 1150 |
| Applications | Microscopy |
| Flow Cytometry | |
| Fluorisa | |
| Fluorescent Western Blotting |
Use the spectra viewer below to compare Alexa Fluor® 680 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.
