Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates
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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Alexa Fluor® 647

Alexa Fluor® 647 Technical Information

Alexa Fluor® 647 - conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. They are brighter than Cy™5 in aqueous mounting media. Alexa Fluor® 647 conjugates are the best choice of far-red emitting dyes for multiple labeling detection with a confocal microscope, and are also widely used in flow cytometry. A significant advantage of long wavelength dyes such as Alexa Fluor® 647 is the low autofluorescence of biological specimens in this region of the spectrum. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. Therefore, Alexa Fluor® 647 is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation and a far-red detector.

Alexa Fluor® 647 has good color separation from DyLight™ 405, Alexa Fluor® 488, and Rhodamine Red-X for effective 4-color imaging.

Alexa Fluor® 647 is available conjugated to:

Excitation Peak (nm) Emission Peak (nm)
Alexa Fluor® 647 651 667

Alexa Fluor® 647 Conjugated Antibodies


Control Reagents Product Code
Alexa Fluor® 647-conjugated ChromPure Chicken IgY (IgG), whole molecule 003-600-003
Alexa Fluor® 647-conjugated ChromPure Goat IgG, F(ab')2 fragment 005-600-006
Alexa Fluor® 647-conjugated ChromPure Goat IgG, whole molecule 005-600-003
Alexa Fluor® 647-conjugated conjugated ChromPure Bovine IgG, whole molecule 001-600-003
Alexa Fluor® 647-conjugated conjugated ChromPure Donkey IgG, F(ab')2 fragment 017-600-006
Alexa Fluor® 647-conjugated conjugated ChromPure Donkey IgG, whole molecule 017-600-003
Alexa Fluor® 647-conjugated conjugated ChromPure Human Albumin 009-600-051
Alexa Fluor® 647-conjugated conjugated ChromPure Human IgG, F(ab')2 fragment 009-600-006
Alexa Fluor® 647-conjugated conjugated ChromPure Human IgG, Fab fragment 009-600-007
Alexa Fluor® 647-conjugated conjugated ChromPure Human IgG, Fc fragment 009-600-008
Alexa Fluor® 647-conjugated conjugated ChromPure Human IgG, whole molecule 009-600-003
Alexa Fluor® 647-conjugated conjugated ChromPure Human IgM (myeloma), whole molecule 009-600-012
Alexa Fluor® 647-conjugated conjugated ChromPure Human Transferrin 009-600-050
Alexa Fluor® 647-conjugated conjugated ChromPure Mouse IgG, F(ab')2 fragment 015-600-006
Alexa Fluor® 647-conjugated conjugated ChromPure Mouse IgG, whole molecule 015-600-003
Alexa Fluor® 647-conjugated conjugated ChromPure Mouse Transferrin 015-600-050
Alexa Fluor® 647-conjugated conjugated ChromPure Rabbit IgG, whole molecule 011-600-003
Alexa Fluor® 647-conjugated conjugated ChromPure Rat IgG, whole molecule 012-600-003
Alexa Fluor® 647-conjugated conjugated ChromPure Rat Transferrin 012-600-050
Alexa Fluor® 647-conjugated conjugated ChromPure Sheep IgG, whole molecule 013-600-003
Streptavidin Product Code
Alexa Fluor® 647-conjugated conjugated Streptavidin 016-600-084

Spectra Viewer

Use the spectra viewer below to compare Alexa Fluor® 647 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.


  Show Excitation and Emission Spectra  

  • Alexa Fluor® fluorescent dyes is a trademark of Life Technologies Corp.
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