Alexa Fluor® 647 Technical Information
Alexa Fluor® 647 - conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. They are brighter than Cy™5 in aqueous mounting media. Alexa Fluor® 647 conjugates are the best choice of far-red emitting dyes for multiple labeling detection with a confocal microscope, and are also widely used in flow cytometry. A significant advantage of long wavelength dyes such as Alexa Fluor® 647 is the low autofluorescence of biological specimens in this region of the spectrum. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. Therefore, Alexa Fluor® 647 is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation and a far-red detector.
Alexa Fluor® 647 has good color separation from DyLight™ 405, Alexa Fluor® 488, and Rhodamine Red-X for effective 4-color imaging.
Alexa Fluor® 647 is available conjugated to:
- Whole IgG Secondary Antibodies
- F(ab')2 Fragment Secondary Antibodies
- Fab Fragment Secondary Antibodies
- FabuLight™ - Fc Specific Fab Fragments for Labeling Primary Antibodies In Solution
- Anti-Fluorescein, Anti-Digoxin, Anti-Biotin, and Anti-HRP Antibodies
- Streptavidin
- ChromPure™ Purified Proteins from Normal Serums
| Excitation Peak (nm) | Emission Peak (nm) | |
|---|---|---|
| Alexa Fluor® 647 | 651 | 667 |
Spectra Viewer
Use the spectra viewer below to compare Alexa Fluor® 647 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.
Alexa Fluor® fluorescent dyes is a trademark of Life Technologies Corp.
