Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates
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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Indocarbocyanine, Cy3

Indocarbocyanine, Cy™3 Technical Information

Cy™3 is brighter, more photostable and gives less background than other orange-red fluorescing dye conjugates. Cy™3 conjugates can be excited maximally at 550 nm, with peak emission at 570 nm. For fluorescence microscopy, Cy™3 can be visualized with traditional tetramethyl rhodamine (TRITC) filter sets, since the excitation and emission spectra are nearly identical to those of TRITC. Cy™3 can be excited to about 50% of maximum with an argon laser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser (543 nm line) or mercury lamp (546 nm line). Cy™3 can be used with green-fluorescing dyes for double labeling. To avoid detection of Cy™3 fluorescence in the green filter set, the emission filter should have a narrow band-pass feature. Cy™3 can also be paired with Alexa Fluor® 647 for multiple labeling.

Cy 3 is available conjugated to:

Excitation Peak (nm) Emission Peak (nm)
Indocarbocyanine, Cy3 550 570

Cy3 Conjugated Antibodies


Control Reagents Product Code
Cy3-conjugated ChromPure Bovine IgG, whole molecule 001-160-003
Cy3-conjugated ChromPure Chicken IgY (IgG), whole molecule 003-160-003
Cy3-conjugated ChromPure Donkey IgG, F(ab')2 fragment 017-160-006
Cy3-conjugated ChromPure Donkey IgG, whole molecule 017-160-003
Cy3-conjugated ChromPure Goat IgG, F(ab')2 fragment 005-160-006
Cy3-conjugated ChromPure Goat IgG, whole molecule 005-160-003
Cy3-conjugated ChromPure Human Albumin 009-160-051
Cy3-conjugated ChromPure Human IgG, F(ab')2 fragment 009-160-006
Cy3-conjugated ChromPure Human IgG, Fab fragment 009-160-007
Cy3-conjugated ChromPure Human IgG, Fc fragment 009-160-008
Cy3-conjugated ChromPure Human IgG, whole molecule 009-160-003
Cy3-conjugated ChromPure Human IgM (myeloma), whole molecule 009-160-012
Cy3-conjugated ChromPure Human Serum IgA, whole molecule 009-160-011
Cy3-conjugated ChromPure Human Transferrin 009-160-050
Cy3-conjugated ChromPure Mouse IgG, F(ab')2 fragment 015-160-006
Cy3-conjugated ChromPure Mouse IgG, Fab fragment 015-160-007
Cy3-conjugated ChromPure Mouse IgG, Fc fragment 015-160-008
Cy3-conjugated ChromPure Mouse IgG, whole molecule 015-160-003
Cy3-conjugated ChromPure Mouse Transferrin 015-160-050
Cy3-conjugated ChromPure Rabbit IgG, whole molecule 011-160-003
Cy3-conjugated ChromPure Rat IgG, whole molecule 012-160-003
Cy3-conjugated ChromPure Rat Transferrin 012-160-050
Cy3-conjugated ChromPure Sheep IgG, whole molecule 013-160-003
Cy3-conjugated ChromPure Syrian Hamster IgG, whole molecule 007-160-003
Streptavidin Product Code
Cy3-conjugated Streptavidin 016-160-084

Spectra Viewer

Use the spectra viewer below to compare Cy3 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.


  • Cy™ is a trademark of GE Healthcare Bio-Sciences Ltd.
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