Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Indocarbocyanine, Cy3

Indocarbocyanine, Cy™3 Technical Information

Cy™3 is brighter, more photostable and gives less background than other orange-red fluorescing dye conjugates. Cy™3 conjugates can be excited maximally at 550 nm, with peak emission at 570 nm. For fluorescence microscopy, Cy™3 can be visualized with traditional tetramethyl rhodamine (TRITC) filter sets, since the excitation and emission spectra are nearly identical to those of TRITC. Cy™3 can be excited to about 50% of maximum with an argon laser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser (543 nm line) or mercury lamp (546 nm line). Cy™3 can be used with green-fluorescing dyes for double labeling. To avoid detection of Cy™3 fluorescence in the green filter set, the emission filter should have a narrow band-pass feature. Cy™3 can also be paired with Alexa Fluor® 647 for multiple labeling.

Cy 3 is available conjugated to:

Cy2, Cy3 and Cy5 Conjugates for Permanent Mounting

Excitation Peak (nm) Emission Peak (nm)
Indocarbocyanine, Cy3 550 570

Spectra Viewer

Use the spectra viewer below to compare Cy3 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.

Read our blog about selecting fluorophores using the Spectra Viewer

Cy™ is a trademark of GE Healthcare Bio-Sciences Ltd.