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Secondary detection: Indirect detection set-up
Secondary detection, also known as indirect detection, has two important advantages over direct detection. These …Read More »
Top 10 Tips for ELISA
Enzyme-linked immunosorbent assay (ELISA) remains one of the most popular immunoassay techniques due to its …Read More »
Considerations for Multiplex Immunofluorescence
A major advantage of fluorescent detection is that it allows for multiplexing. Depending on the …Read More »
Tissue Clearing Techniques
The term ‘tissue clearing’ describes a collection of techniques used for making large, fixed biological …Read More »
Assay Setup: Sandwich ELISA for Allergy
ELISA is a widely used technique for detecting and quantifying one or more specific proteins …Read More »
ELISA Guide; Part 4: Troubleshooting
Overview No Signal or Weak Signal High Background Poor Reproducibility Between Plates Poor Reproducibility Between …Read More »
AffiniPure-VHH™ secondary antibodies for flow cytometry
Flow cytometry is a technique used to analyze individual cells in suspension. It uses a …Read More »ELISA Guide; Part 3: ELISA Optimization
Overview Optimization Controls and Standards Validation Spike and Recovery Dilutional Linearity Parallelism Data Analysis Optimization …Read More »
NEW: 4 part ELISA Guide
An Introduction to Surface Plasmon Resonance
Surface Plasmon Resonance (SPR) is a label-free optical biosensing technique that allows researchers to measure …Read More »
ELISA Guide; Part 2: The ELISA Protocol
Overview Key Stages in the ELISA Protocol Capture Sample Preparation Analyte-Specific Antibody Considerations Secondary Antibody …Read More »
Western Blotting Guide
ELISA Guide; Part 1: Introduction to ELISA, Formats and Signal Amplification
Introduction to ELISA Enzyme-linked immunosorbent assay (ELISA) was first described in 1971 when it was …Read More »
An Introduction to Expansion Microscopy
Expansion microscopy is a relatively new imaging technique that was developed to improve the resolution …Read More »
Detecting ScFVs – Considerations for optimal detection
Single chain variable fragments (scFvs) are commonly used in CAR-T (chimeric antigen receptor T-Cell) cell …Read More »
Western blotting guide: Part 8, Visualization
Visualization of the target proteins is the object of a western blot. There are a …Read More »
Vial fill size, protein concentration, and reconstitution volumes
We are sometimes asked about the discrepancy between the protein concentration recorded, the recommended volume …Read More »Troubleshooting: Using Anti-Light Chain Antibodies
Anti-light chain antibodies are commonly used for Western blotting (WB) after Immunoprecipitation (IP) when detecting …Read More »
Western blotting guide: Part 7, Membrane Washing
Washing removes unbound or aggregated proteins present on the blot as well as unbound reagents …Read More »Western blotting guide: Part 6, Secondary Antibodies
The secondary antibody detects the primary antibody, typically conjugated to a reporter molecule it enables …Read More »
Western blotting guide: Part 5, Primary Antibodies
Primary antibodies are used to detect the protein of interest. Part 5 of the Western …Read More »
Western blotting guide: Part 4, Membrane blocking
Blocking is essential to prevent non-specific interactions between the transferred proteins, the membrane and the …Read More »
Western blotting guide: Part 3, Electroblotting – Protein Transfer
Electrophoresis allows the proteins separated by SDS-PAGE to be transfered from the gel onto a …Read More »
Western blotting guide: Part 2, Protein separation by SDS-PAGE
Once prepared, the sample proteins are separated by PAGE (polyacrylamide gel electrophoresis), which may be …Read More »
Affinity Vs Avidity
Affinity and avidity are terms used to describe the strength of the bond between an …Read More »Western blotting guide: Part 1, Introduction and Sample Preparation
In 1979, Towbin et al. first detailed the process of immunoblotting – the separation of …Read More »
Immunoglossary
Antibodies are such critical reagents for scientific research that a unique language has been coined …Read More »Ten Top Tips for Multiple Labeling
. Multiplexed immunoassays offer many advantages. These include more data points per sample, higher throughput, …Read More »
High-Affinity Nanobodies: Potential Therapeutics for SARS-CoV-2
Of the seven types of coronavirus known to infect humans, severe acute respiratory syndrome coronavirus …Read More »
Ten Top Tips for Flow Cytometry
Flow cytometry is a technique for analyzing individual cells in suspension. It uses a stream …Read More »
Antibodies – From Immune Weapons to Essential Tools for Research
Antibodies are complex proteins produced by B-cells during the adaptive immune response. They recognize and …Read More »Selecting Fluorophores for Antibody-based Research
Fluorophores are essential tools for scientific research. They are widely used for immunoassay techniques such …Read More »
Optimize Nanobody Development
Are you one of the many labs working to develop VHH antibodies (nanobodies)? Our COO …Read More »
Western blotting – 10 tips for better blots
Western blotting is a technique used to confirm the presence of target proteins and peptides …Read More »
An Introduction to Secondary Antibodies
Antibodies are invaluable to scientific research, diagnostics, and therapeutics. These simple yet powerful scientific tools …Read More »
Fluorophore selection and panel building – Spectra Viewer
Fluorescent probes or fluorophores (fluorescent dyes or proteins) are coupled to a secondary antibody or …Read More »Solutions for Species on Species Experiments
When using primary antibodies derived from the same species as the sample material (species on …Read More »
Cross-adsorbed secondary antibodies and cross-reactivity
Immunoglobulins from different species share similar structures. Secondary antibodies raised against one species are likely …Read More »
A guide to selecting control and blocking reagents.
Experimental protocols using immunotechniques can often be improved through the optimal use of blocking reagents, diluents, and …Read More »
Western blot troubleshooting guide!
Western blotting is a staple technique of the molecular biology lab. The robust nature of …Read More »
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