Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates
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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

Rating: 5.0

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Alexa Fluor® 680 and 790

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Alexa Fluor® 680 and 790 Technical Information

Alexa Fluor® 680 and Alexa Fluor® 790 conjugates are used for very sensitive Western blots, ELISAs, and multiplexing arrays. Alexa Fluor® 680 conjugates are excited with a peak around 684 nm and fluoresce with a peak around 702 nm. Alexa Fluor® 790 conjugates are excited with a peak around 792 nm and fluoresce at a peak around 803 nm. They are the best choice for highly sensitive single or double labeling with fluorescence imaged in a LI-COR Odyssey® imager.

Dye
Laser line (nm)
Common filter set
Excitation (nm)
Emission (nm)
Extinction co-efficient
Quantum Yield (Φ)
Molecular Weight (kDa)
Applications
Alexa Fluor® 680 633 684 702 1.4 N/A 1150 Microscopy, Flow Cytometry, Fluorisa, Fluorescent Western Blotting
Alexa Fluor® 790 792 803 1.4 N/A 1750 Microscopy, Flow Cytometry, Fluorisa, Fluorescent Western Blotting
Dye Alexa Fluor® 680
Laser line (nm) 633
Common filter set
Excitation (nm) 684
Emission (nm) 702
Extinction co-efficient 1.4
Quantum Yield (Φ) N/A
Molecular Weight (kDa) 1150
Applications Microscopy
Flow Cytometry
Fluorisa
Fluorescent Western Blotting
Dye Alexa Fluor® 790
Laser line (nm)
Common filter set
Excitation (nm) 792
Emission (nm) 803
Extinction co-efficient 1.4
Quantum Yield (Φ) N/A
Molecular Weight (kDa) 1750
Applications Microscopy
Flow Cytometry
Fluorisa
Fluorescent Western Blotting

Alexa Fluor® 680 & 790 Conjugated Antibodies


Control Reagents Product Code
Alexa Fluor® 680-conjugated ChromPure Donkey IgG, whole molecule 017-620-003
Alexa Fluor® 790-conjugated ChromPure Donkey IgG, whole molecule 017-650-003
Alexa Fluor® 680-conjugated ChromPure Goat IgG, whole molecule 005-620-003
Alexa Fluor® 790-conjugated ChromPure Goat IgG, whole molecule 005-650-003
Alexa Fluor® 680-conjugated ChromPure Mouse IgG, whole molecule 015-620-003
Alexa Fluor® 790-conjugated ChromPure Mouse IgG, whole molecule 015-650-003
Streptavidin Product Code
Alexa Fluor® 680-conjugated Streptavidin 016-620-084
Alexa Fluor® 790-conjugated Streptavidin 016-650-084

Spectra Viewer

Use the spectra viewer below to compare Alexa Fluor® 680 and 790 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.


  Show Excitation and Emission Spectra  

  • Alexa Fluor® fluorescent dyes is a trademark of Life Technologies Corp.
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