Alexa Fluor® 680 and Alexa Fluor® 790 conjugates are used for very sensitive Western blots, ELISAs, and multiplexing arrays. Alexa Fluor® 680 conjugates are excited with a peak around 684 nm and fluoresce with a peak around 702 nm. Alexa Fluor® 790 conjugates are excited with a peak around 792 nm and fluoresce at a peak around 803 nm. They are the best choice for highly sensitive single or double labeling with fluorescence imaged in a LI-COR Odyssey® imager.
Excitation and emission spectra of Alexa Fluor® 680 (left) and Alexa Fluor® 790 (right) conjugated affinity-purified secondary antibodies, streptavidin, and purified proteins. Peak heights have been normalized, spectra were obtained with an M-Series spectrophotometer system from Photon Technology International, Inc. Values are approximate, actual values may vary depending on the spectrofluorometer used in each laboratory.
|Excitation Peak||Emission Peak (nm)|
|Alexa Fluor® 680||684||702|
|Alexa Fluor® 790||792||803|
Alexa Fluor® fluorescent dyes are a trademark of Life Technologies Corp.