Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Multiple Labeling

With BV421™ & BV480™

  • Combine with AF488, RR-X, and AF647 for 5-color imaging
  • Compatible with commonly used filter sets
  • Switch nuclear stain from DAPI to DRAQ5 for additional labeling options

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Alexa Fluor® 680 and 790

Alexa Fluor® 680 and 790 Technical Information

Alexa Fluor® 680 and Alexa Fluor® 790 conjugates are used for very sensitive Western blots, ELISAs, and multiplexing arrays. Alexa Fluor® 680 conjugates are excited with a peak around 684 nm and fluoresce with a peak around 702 nm. Alexa Fluor® 790 conjugates are excited with a peak around 792 nm and fluoresce at a peak around 803 nm. They are the best choice for highly sensitive single or double labeling with fluorescence imaged in a LI-COR Odyssey® imager.

Alexa Fluor 680 SpectraAlexa Fluor 790 Spectra Excitation and emission spectra of Alexa Fluor® 680 (left) and Alexa Fluor® 790 (right) conjugated affinity-purified secondary antibodies, streptavidin, and purified proteins. Peak heights have been normalized, spectra were obtained with an M-Series spectrophotometer system from Photon Technology International, Inc. Values are approximate, actual values may vary depending on the spectrofluorometer used in each laboratory.

Excitation Peak Emission Peak (nm)
Alexa Fluor® 680 684 702
Alexa Fluor® 790 792 803

Alexa Fluor® fluorescent dyes are a trademark of Life Technologies Corp.