Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates
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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Alexa Fluor® 488

Alexa Fluor® 488 Technical Information

Alexa Fluor® 488 - conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media (see Cy™2 for more information).

Alexa Fluor® 488 has good color separation from DyLight™ 405, Rhodamine Red-X, and Alexa Fluor® 647 for effective 4‑color imaging.

IF image of Alpha tubulin visualised with JIR Alexa Fluor® 488-conjugated secondary antibodies
Alpha tubulin visualised with 115-545-205 (Alexa Fluor® 488 Goat Anti-Mouse IgG, Fcγ subclass 1 specific).
Image by David Robertson, ICR.

Alexa Fluor® 488 is available conjugated to:

Excitation Peak (nm) Emission Peak (nm)
Alexa Fluor® 488 493 519

Alexa Fluor® 488 Conjugated Antibodies


Control Reagents Product Code
Alexa Fluor® 488-conjugated ChromPure Bovine IgG, whole molecule 001-540-003
Alexa Fluor® 488-conjugated ChromPure Chicken IgY (IgG), whole molecule 003-540-003
Alexa Fluor® 488-conjugated ChromPure Donkey IgG, F(ab')2 fragment 017-540-006
Alexa Fluor® 488-conjugated ChromPure Donkey IgG, whole molecule 017-540-003
Alexa Fluor® 488-conjugated ChromPure Goat IgG, F(ab')2 fragment 005-540-006
Alexa Fluor® 488-conjugated ChromPure Goat IgG, whole molecule 005-540-003
Alexa Fluor® 488-conjugated ChromPure Human Albumin 009-540-051
Alexa Fluor® 488-conjugated ChromPure Human IgG, F(ab')2 fragment 009-540-006
Alexa Fluor® 488-conjugated ChromPure Human IgG, Fab fragment 009-540-007
Alexa Fluor® 488-conjugated ChromPure Human IgG, Fc fragment 009-540-008
Alexa Fluor® 488-conjugated ChromPure Human IgG, whole molecule 009-540-003
Alexa Fluor® 488-conjugated ChromPure Human IgM (myeloma), whole molecule 009-540-012
Alexa Fluor® 488-conjugated ChromPure Human Transferrin 009-540-050
Alexa Fluor® 488-conjugated ChromPure Mouse IgG, F(ab')2 fragment 015-540-006
Alexa Fluor® 488-conjugated ChromPure Mouse IgG, whole molecule 015-540-003
Alexa Fluor® 488-conjugated ChromPure Mouse Transferrin 015-540-050
Alexa Fluor® 488-conjugated ChromPure Rabbit IgG, whole molecule 011-540-003
Alexa Fluor® 488-conjugated ChromPure Rat IgG, whole molecule 012-540-003
Alexa Fluor® 488-conjugated ChromPure Rat Transferrin 012-540-050
Alexa Fluor® 488-conjugated ChromPure Sheep IgG, whole molecule 013-540-003
Streptavidin Product Code
Alexa Fluor® 488-conjugated Streptavidin 016-540-084

Spectra Viewer

Use the spectra viewer below to compare Alexa Fluor® 488 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.


  Show Excitation and Emission Spectra  

  • Alexa Fluor® fluorescent dyes is a trademark of Life Technologies Corp.
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