"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio University
Alpha tubulin visualised with 115-545-205 (Alexa Fluor® 488 Goat Anti-Mouse IgG, Fcγ subclass 1 specific).
Image by David Robertson, ICR.
Alexa Fluor® 488 - conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media (see Cy™2 for more information).
Alexa Fluor® 488 has good color separation from DyLight™ 405, Rhodamine Red-X, and Alexa Fluor® 647 for effective 4‑color imaging.
Alexa Fluor® 488 is available conjugated to:
| Excitation Peak | Emission Peak (nm) | |
|---|---|---|
| Alexa Fluor® 488 | 493 | 519 |
Use the spectra viewer below to compare Alexa Fluor® 488 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.
Read our blog about selecting fluorophores using the Spectra Viewer
Alexa Fluor® fluorescent dyes is a trademark of Life Technologies Corp.
