"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio UniversityRating: 5.0
Alexa Fluor® 488 - conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media (see Cy™2 for more information).
Alexa Fluor® 488 is a versatile dye and can be combined with different dyes for effective 4 color immunofluorescence.
Alexa Fluor® 488 is available conjugated to:
Dye |
Laser line (nm) |
Common filter set |
Excitation (nm) |
Emission (nm) |
Extinction co-efficient |
Quantum Yield (Φ) |
Molecular Weight (kDa) |
Applications |
|---|---|---|---|---|---|---|---|---|
| Alexa Fluor® 488 | 488 | 530/30 | 493 | 519 | 1.4 | 0.92 | 643 | Microscopy, SRM, Flow Cytometry, Fluorisa, Fluorescent Western Blotting |
| Dye | Alexa Fluor® 488 |
|---|---|
| Laser line (nm) | 488 |
| Common filter set | 530/30 |
| Excitation (nm) | 493 |
| Emission (nm) | 519 |
| Extinction co-efficient | 1.4 |
| Quantum Yield (Φ) | 0.92 |
| Molecular Weight (kDa) | 643 |
| Applications | Microscopy |
| SRM | |
| Flow Cytometry | |
| Fluorisa | |
| Fluorescent Western Blotting |
| Control Reagents | Product Code |
|---|---|
| Alexa Fluor® 488-conjugated ChromPure Bovine IgG, whole molecule | 001-540-003 |
| Alexa Fluor® 488-conjugated ChromPure Chicken IgY (IgG), whole molecule | 003-540-003 |
| Alexa Fluor® 488-conjugated ChromPure Donkey IgG, F(ab')2 fragment | 017-540-006 |
| Alexa Fluor® 488-conjugated ChromPure Donkey IgG, whole molecule | 017-540-003 |
| Alexa Fluor® 488-conjugated ChromPure Goat IgG, F(ab')2 fragment | 005-540-006 |
| Alexa Fluor® 488-conjugated ChromPure Goat IgG, whole molecule | 005-540-003 |
| Alexa Fluor® 488-conjugated ChromPure Human Albumin | 009-540-051 |
| Alexa Fluor® 488-conjugated ChromPure Human IgG, F(ab')2 fragment | 009-540-006 |
| Alexa Fluor® 488-conjugated ChromPure Human IgG, Fab fragment | 009-540-007 |
| Alexa Fluor® 488-conjugated ChromPure Human IgG, Fc fragment | 009-540-008 |
| Alexa Fluor® 488-conjugated ChromPure Human IgG, whole molecule | 009-540-003 |
| Alexa Fluor® 488-conjugated ChromPure Human IgM (myeloma), whole molecule | 009-540-012 |
| Alexa Fluor® 488-conjugated ChromPure Human Transferrin | 009-540-050 |
| Alexa Fluor® 488-conjugated ChromPure Mouse IgG, F(ab')2 fragment | 015-540-006 |
| Alexa Fluor® 488-conjugated ChromPure Mouse IgG, whole molecule | 015-540-003 |
| Alexa Fluor® 488-conjugated ChromPure Mouse Transferrin | 015-540-050 |
| Alexa Fluor® 488-conjugated ChromPure Rabbit IgG, whole molecule | 011-540-003 |
| Alexa Fluor® 488-conjugated ChromPure Rat IgG, whole molecule | 012-540-003 |
| Alexa Fluor® 488-conjugated ChromPure Rat Transferrin | 012-540-050 |
| Alexa Fluor® 488-conjugated ChromPure Sheep IgG, whole molecule | 013-540-003 |
| Streptavidin | Product Code |
| Alexa Fluor® 488-conjugated Streptavidin | 016-540-084 |
Use the spectra viewer below to compare Alexa Fluor® 488 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.
