"We only use secondary antibodies from Jackson ImmunoResearch whenever possible! Customer service is also excellent and the price point for their products is very competitive."
Elizabeth Soberg, University of Washington
Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recomended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media.
Alexa Fluor® 488 has good color separation from DyLight 405, Rhodamine Red-X, and Alexa Fluor® 647 for effective 4-color imaging.
Excitation and emission spectra of Alexa Fluor® 488-conjugated affinity-purified secondary antibodies, streptavidin, and purified proteins. Peak heights have been normalized, spectra were obtained with an M-Series spectrophotometer system from Photon Technology International, Inc. Values are approximate, actual values may vary depending on the spectrofluorometer used in each laboratory.
Emission spectra for optimal 4-color imaging using DyLight 405 (blue), Alexa Fluor® 488 (green), Rhodamine Red-X (red), and Alexa Fluor® 647 (purple).
| Excitation Peak | Emission Peak (nm) | |
|---|---|---|
| Alexa Fluor® 488 | 493 | 519 |
GFAPalpha DAPI primary astrocyte culture 1. Primary mixed glial culture derived from P0-P3 mouse pups, stained for GFAP C-term (Santa Cruz sc-6170) and donkey-anti-goat Alexa Fluor® 488 (Jackson ImmunoResearch 705-546-147). Authors: Carlyn Martina-Mamber, Elly M. Hol.
Alexa Fluor® fluorescent dyes are a trademark of Life Technologies Corp.
