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Alexa Fluor® 488
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Alexa Fluor® 488 Technical Information
Alexa Fluor® 488 - conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media (see Cy™2 for more information ).
Alexa Fluor® 488 is a versatile dye and can be combined with different dyes for effective 4 color immunofluorescence.
Alpha tubulin visualised with 115-545-205 (Alexa Fluor® 488 Goat Anti-Mouse IgG, Fcγ subclass 1 specific). Image by David Robertson, ICR.
Alexa Fluor® 488 is available conjugated to:
Dye
Laser line (nm)
Common filter set
Excitation (nm)
Emission (nm)
Extinction co-efficient
Quantum Yield (Φ)
Molecular Weight (Da)
Applications
Alexa Fluor® 488
488
530/30
493
519
1.4
0.92
643
Microscopy, SRM, Flow Cytometry, Fluorisa, Fluorescent Western Blotting
Dye
Alexa Fluor® 488
Laser line (nm)
488
Common filter set
530/30
Excitation (nm)
493
Emission (nm)
519
Extinction co-efficient
1.4
Quantum Yield (Φ)
0.92
Molecular Weight (Da)
643
Applications
Microscopy
SRM
Flow Cytometry
Fluorisa
Fluorescent Western Blotting
Alexa Fluor® 488 Conjugated Antibodies
Specificity
Conjugate
Target
Host
Format
Product Description
Product Code
Alexa Fluor® 488 AffiniPure® Goat Anti-Alpaca IgG (H+L)
(min X Bov, Hu, Ms , Rb, Rat Sr Prot)
128-545-160 Alexa Fluor® 488 AffiniPure® Goat Anti-Alpaca IgG (H+L)
128-545-003 Alexa Fluor® 488 AffiniPure® Goat Anti-Alpaca IgG, subclasses 2+3 specific
(min X Bov, Hu, Ms, Rb, Rat Sr Prot)
128-545-229 Alexa Fluor® 488 AffiniPure® Fab Fragment Goat Anti-Alpaca IgG, VHH domain
(min X Bov Sr Prot)
128-547-232 Alexa Fluor® 488 AffiniPure® Goat Anti-Alpaca IgG, VHH domain
(min X Bov Sr Prot)
128-545-232
Control Reagents
Product Code
Alexa Fluor® 488-conjugated ChromPure Bovine IgG, whole molecule
001-540-003
Alexa Fluor® 488-conjugated ChromPure Chicken IgY (IgG), whole molecule
003-540-003
Alexa Fluor® 488-conjugated ChromPure Donkey IgG, F(ab')2 fragment
017-540-006
Alexa Fluor® 488-conjugated ChromPure Donkey IgG, whole molecule
017-540-003
Alexa Fluor® 488-conjugated ChromPure Goat IgG, F(ab')2 fragment
005-540-006
Alexa Fluor® 488-conjugated ChromPure Goat IgG, whole molecule
005-540-003
Alexa Fluor® 488-conjugated ChromPure Human Albumin
009-540-051
Alexa Fluor® 488-conjugated ChromPure Human IgG, F(ab')2 fragment
009-540-006
Alexa Fluor® 488-conjugated ChromPure Human IgG, Fab fragment
009-540-007
Alexa Fluor® 488-conjugated ChromPure Human IgG, Fc fragment
009-540-008
Alexa Fluor® 488-conjugated ChromPure Human IgG, whole molecule
009-540-003
Alexa Fluor® 488-conjugated ChromPure Human IgM (myeloma), whole molecule
009-540-012
Alexa Fluor® 488-conjugated ChromPure Human Transferrin
009-540-050
Alexa Fluor® 488-conjugated ChromPure Mouse IgG, F(ab')2 fragment
015-540-006
Alexa Fluor® 488-conjugated ChromPure Mouse IgG, whole molecule
015-540-003
Alexa Fluor® 488-conjugated ChromPure Mouse Transferrin
015-540-050
Alexa Fluor® 488-conjugated ChromPure Rabbit IgG, whole molecule
011-540-003
Alexa Fluor® 488-conjugated ChromPure Rat IgG, whole molecule
012-540-003
Alexa Fluor® 488-conjugated ChromPure Rat Transferrin
012-540-050
Alexa Fluor® 488-conjugated ChromPure Sheep IgG, whole molecule
013-540-003
Streptavidin
Product Code
Alexa Fluor® 488-conjugated Streptavidin
016-540-084
Spectra Viewer
Use the spectra viewer below to compare Alexa Fluor® 488 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.
Show Excitation and Emission Spectra
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