Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates
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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Alexa Fluor® 568

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NEW Alexa Fluor® 568 conjugate from Jackson ImmunoResearch

Access the full spectrum - flexible panel design

Secondary antibody conjugates designed for multiple labeling

NEW Alexa Fluor® 555 and Alexa Fluor® 568 dyes are conjugated to Donkey and Goat host species secondary antibodies designed with multiple labeling in mind as they are minimally cross-reactive to many common species. Combine Alexa Fluor® 555 and 568 with our full range of JIR Alexa Fluor® conjugated secondary antibodies to design labeling experiments that access the full spectrum!

Table 1: Alexa Fluor® dyes available conjugated to Jackson ImmunoResearch products.
Alexa Fluor® Excitation Peak (nm) Emission Peak (nm)
Alexa Fluor® 488 493 519
NEW! Alexa Fluor® 555 552 572
NEW! Alexa Fluor® 568 577 602
Alexa Fluor® 594 591 614
Alexa Fluor® 647 651 667
Alexa Fluor® 680 684 702
Alexa Fluor® 790 792 803
Excitation and emission spectra for Alexa Fluor® dyes available conjugated to Jackson ImmunoResearch products
Figure 1: Excitation and emission spectra for Alexa Fluor® dyes available conjugated to Jackson ImmunoResearch products

Alexa Fluor® dye conjugates are the industry standard for brightness and usability. They are highly water-soluble and are fluorescent across a wide pH range. They offer superb brightness and photostability with minimal background and are compatible with the most common excitation sources. Jackson ImmunoResearch introduces two new dyes to its portfolio, Alexa Fluor® 555 and 568, offering you two more choices in the orange channel for more flexible panel design!

Excitation and Emission spectra for Alexa Fluor® 555 and 568
Figure 2: Excitation and Emission spectra for Alexa Fluor® 555 and 568

Alexa Fluor® 568

Dye Alexa Fluor® 568
Laser line (nm) 568
Common filter set
Excitation (nm) 577
Emission (nm) 602
Extinction co-efficient 1.4
Quantum Yield (Φ) 0.69
Molecular Weight (kDa) 792
Applications Microscopy
Flow Cytometry
Fluorescent Western Blotting
Dye Laser line (nm) Excitation (nm) Emission (nm) Extinction co-efficient Quantum Yield (Φ) Molecular Weight (kDa) Applications
Alexa Fluor® 568 568 577 602 1.4 0.69 792 Microscopy, SRM, Flow Cytometry, Fluorisa, Fluorescent Western Blotting

Alexa Fluor® 568 conjugated antibodies absorb light maximally around 577 nm and fluoresce with a peak around 602 nm. Alexa Fluor® 568 dyes are bright, orange-fluorescent dyes with excitation ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser. Alexa Fluor® 568 dyes are pH-insensitive over a wide molar range. Alexa Fluor® 568 is a bright and photostable orange-red dye that matches well with Red Fluorescent Protein (RFP) filter sets and may be used in stochastic optical reconstruction microscopy (STORM)—as a reporter in dSTORM (Goossen-Schmidt et al., 2020).

Figure 3: Indirect four-color immunostaining of Human epithelial (HEp-2) cells. Cellular proliferation visualized in green, using Rabbit A-Ki-67 followed by Alexa Fluor® 488-conjugated Donkey Anti-Rabbit (H+L) (711-545-152). Microtubules visualized in yellow, using Mouse anti-α-tubulin followed by AlexaFluor® 568 conjugated Donkey Anti-Mouse IgG (H+L) (715-575-150). Cell-to-cell contact visualized in red using Goat Anti- E-Cadherin, followed by Alexa Fluor® 647-conjugated Donkey Anti-Goat IgG (H+L) (705-605-147). Blue - DAPI Nuclear Stain.

Alexa Fluor® 568 is available conjugated to:

Alexa Fluor® 568 Conjugated Antibodies

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  • Alexa Fluor® fluorescent dyes are a trademark of Life Technologies Corp and are provided under an agreement between Life Technologies Corp and Jackson ImmunoResearch Laboratories, Inc and the manufacture, use, sale, or import of these dyes is sold pursuant to a license from Life Technologies Corp for use of its fluorescent dye technology.