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"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio University
We have produced Alpaca secondary antibodies anti-Mouse IgG (H+L), anti-Rabbit IgG (H+L), and anti-Human IgG (H+L). All have minimal cross reactivity with bovine serum proteins to avoid background from BSA blocking steps and cell culture components. Additionally they are cross-adsorbed against two of the following species - Human, Mouse and Rabbit - depending on their specificity.
Brilliant Violet 421™ and Brilliant Violet 480™ conjugated secondary antibodies allow you to add more colors to your multiple labeling assays in the violet-blue region of the spectrum.
FabuLight antibodies are Fab fragment secondary antibodies specific to the Fc region of IgG or IgM primary antibodies. They provide a time saving alternative to sequential incubation flow cytometry and immunohistochemistry procedures, without compromising the active site of the primary antibody.
In an effort to make super resolution microscopy accessible to a wide variety of scientists, Jackson ImmunoResearch continues to dedicate itself to collaborate with researchers and internally develop novel labeled secondary antibodies optimized for this new frontier.
Antibodies conjugated with far-red- and Infrared-emitting dyes are more sensitive than those with dyes emitting visible light due to low fluorescence quenching of the conjugates, high extinction coefficients of the dyes, and low background autofluorescence.
Cyanine dyes are brighter in the non-polar environment than in an aqueous medium, resulting in less aquisition time than the Alexa Fluor® dyes, even though those dyes are brighter in aqueous mounting media.
This offering now includes our AffiniPure antibodies and other proteins conjugated with Alexa Fluor® 647, Alexa Fluor® 594, and the very bright Alexa Fluor® 488.
A 50 kDa protein may be detected on blots without interference from the precipitated IgG in the same band by incubation with its unlabeled primary antibody followed by labeled anti-IgG, Light Chain Specific.
It is possible to perform effective 4-color imaging with good color separation, good photostability, and high sensitivity in both aqueous and permanent mounting media. The combination of DyLight 405, Alexa Fluor® 488, Rhodamine Red-X, and Alexa Fluor® 647 provides for maximum color separation.
PerCP conjugates of highly adsorbed secondary antibodies are offered to label unconjugated primary antibodies, and PerCP-streptavidin is offered to label biotinylated primary or secondary antibodies.