"Without question, I can always turn to Jackson ImmunoResearch secondary antibodies for ECL detection of any primary antibody! They produce great signal at low dilutions."
W Thompson, OSHU
In an effort to make super resolution microscopy accessible to a wide variety of scientists, Jackson ImmunoResearch continues to dedicate itself to collaborate with researchers and internally develop novel labeled secondary antibodies optimized for this new frontier.
Antibodies conjugated with far-red- and Infrared-emitting dyes are more sensitive than those with dyes emitting visible light due to low fluorescence quenching of the conjugates, high extinction coefficients of the dyes, and low background autofluorescence.
Cyanine dyes are brighter in the non-polar environment than in an aqueous medium, resulting in less aquisition time than the Alexa Fluor® dyes, even though those dyes are brighter in aqueous mounting media.
This offering now includes our AffiniPure antibodies and other proteins conjugated with Alexa Fluor® 647, Alexa Fluor® 594, and the very bright Alexa Fluor® 488.
A 50 kDa protein may be detected on blots without interference from the precipitated IgG in the same band by incubation with its unlabeled primary antibody followed by labeled anti-IgG, Light Chain Specific.
It is possible to perform effective 4-color imaging with good color separation, good photostability, and high sensitivity in both aqueous and permanent mounting media. The combination of DyLight 405, Alexa Fluor® 488, Rhodamine Red-X, and Alexa Fluor® 647 provides for maximum color separation.
PerCP conjugates of highly adsorbed secondary antibodies are offered to label unconjugated primary antibodies, and PerCP-streptavidin is offered to label biotinylated primary or secondary antibodies.